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Related Concept Videos

Gene Therapy00:59

Gene Therapy

Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be inserted. The...
Gene Therapy00:59

Gene Therapy

Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be inserted. The...
Microorganisms in Medicine and Therapeutics01:29

Microorganisms in Medicine and Therapeutics

Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.

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Related Experiment Video

Updated: May 10, 2026

Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform
08:02

Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform

Published on: November 7, 2013

Novel gene delivery systems.

Steffy B Manjila1, Jomon N Baby, Elambilan N Bijin

  • 1College of Pharmaceutical Sciences, Government Medical College, Thiruvananthapuram, Kerala, India.

International Journal of Pharmaceutical Investigation
|June 27, 2013
PubMed
Summary

Gene therapy shows promise for chronic diseases, but effective gene delivery remains a challenge. Researchers are developing advanced nonviral gene delivery systems to overcome the toxicity and limitations of viral methods.

Keywords:
Dendrimersgene therapyliposomesnonviral gene deliveryviral gene delivery

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Expression of Fluorescent Fusion Proteins in Murine Bone Marrow-derived Dendritic Cells and Macrophages

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Last Updated: May 10, 2026

Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform
08:02

Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform

Published on: November 7, 2013

Expression of Fluorescent Fusion Proteins in Murine Bone Marrow-derived Dendritic Cells and Macrophages
09:07

Expression of Fluorescent Fusion Proteins in Murine Bone Marrow-derived Dendritic Cells and Macrophages

Published on: October 30, 2018

Area of Science:

  • Biomedical Sciences
  • Pharmaceutical Sciences
  • Medical Sciences

Background:

  • Gene therapy offers potential for treating chronic diseases like cancer and genetic disorders.
  • Current gene therapy applications are limited by inefficient gene introduction methods.
  • Effective gene delivery systems are crucial for successful gene therapy outcomes.

Purpose of the Study:

  • To review recent advancements in gene delivery systems for gene therapy.
  • To explore the relevancy of novel gene delivery systems.
  • To compare viral and nonviral gene delivery methods.

Main Methods:

  • Exploration of viral gene transfer methods, noting their high efficiency but also toxicity.
  • Investigation of nonviral gene delivery systems, including polymer-based carriers, dendrimers, and physical methods (electroporation, microinjection).
  • Analysis of the advantages of nonviral systems, such as reduced immunogenicity, toxicity, and feasibility of repeated administration.

Main Results:

  • Viral vectors offer high efficiency, long-term expression, stability, and integrity but pose toxicity risks.
  • Nonviral gene delivery systems are being developed to overcome the safety concerns associated with viral vectors.
  • Promising nonviral systems include polymer-based carriers, dendrimers, and physical methods, offering improved safety profiles.

Conclusions:

  • Development of safe and efficient gene delivery systems is paramount for advancing gene therapy.
  • Nonviral gene delivery systems present a promising alternative to viral vectors, balancing efficiency with reduced toxicity.
  • Continued research into novel gene delivery systems is essential for realizing the full potential of gene therapy in treating complex diseases.