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Related Concept Videos

Separation of Sister Chromatids02:17

Separation of Sister Chromatids

At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...

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Related Experiment Video

Updated: May 10, 2026

Development of Targeting Induced Local Lesions IN Genomes (TILLING) Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis
08:36

Development of Targeting Induced Local Lesions IN Genomes (TILLING) Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis

Published on: July 16, 2019

Separating homeologs by phasing in the tetraploid wheat transcriptome.

Ksenia V Krasileva, Vince Buffalo, Paul Bailey

    Genome Biology
    |June 27, 2013
    PubMed
    Summary

    Developing a new bioinformatics workflow for tetraploid wheat, this study enhances transcriptome assembly and separates homoeologous genomes. This approach improves gene model prediction for wheat research.

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    Isolation and Transcriptome Analysis of Plant Cell Types
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    Isolation and Transcriptome Analysis of Plant Cell Types

    Published on: April 7, 2023

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    Last Updated: May 10, 2026

    Development of Targeting Induced Local Lesions IN Genomes (TILLING) Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis
    08:36

    Development of Targeting Induced Local Lesions IN Genomes (TILLING) Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis

    Published on: July 16, 2019

    Isolation and Transcriptome Analysis of Plant Cell Types
    08:53

    Isolation and Transcriptome Analysis of Plant Cell Types

    Published on: April 7, 2023

    Area of Science:

    • Plant Genomics
    • Bioinformatics
    • Transcriptomics

    Background:

    • Tetraploid wheat's homoeologous genomes pose challenges for transcriptome assembly.
    • A specialized bioinformatics workflow was developed to optimize assembly and separate merged homoeologs.

    Purpose of the Study:

    • To develop and evaluate a bioinformatics workflow for de novo transcriptome assembly in tetraploid wheat.
    • To improve the separation of homoeologous genomes in wheat transcriptome data.

    Main Methods:

    • Utilized a multiple k-mer assembly strategy for transcriptome assembly.
    • Employed a post-assembly pipeline including polymorphism identification and SNP phasing for homoeolog separation.
    • Compared assemblies with a benchmark set of 13,472 bread wheat cDNAs.

    Main Results:

    • Assembled 489 million reads into 140,118 contigs, covering 96% of benchmark cDNAs.
    • The multiple k-mer strategy improved full-length cDNA assembly by 22%.
    • Achieved 98.7% accuracy in separating single-nucleotide polymorphisms (SNPs) between homoeologs using phasing.

    Conclusions:

    • Multiple k-mer assembly strategies are more beneficial for tetraploid wheat than diploid wheat.
    • Phasing approaches effectively separate closely related homoeologous genomes in tetraploid wheat.
    • The predicted proteome and gene models offer valuable resources for wheat genomics research.