Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Global Study on Newborn HOspitalization and Parental Experiences (HOPE): rationale and design of a multinational cross-sectional mixed-methods online survey.

BMJ paediatrics open·2026
Same author

Telemedicine adoption in Ecuador: an assessment of physician perceptions and knowledge towards its benefits and limitations.

mHealth·2025
Same author

Multipotent adult progenitor cell therapy: Effect of timing and frequency on lung health in preterm lambs during inflammation.

Pharmacological research·2025
Same author

Anesthesia and mitochondria: balancing toxicity and protection through emerging therapeutic strategies.

Journal of anesthesia·2025
Same author

Perspectives on Implementing European Newborn Health Standards: Insights From Parent Representatives and Healthcare Professionals.

Acta paediatrica (Oslo, Norway : 1992)·2025
Same author

Study protocol for the development and pilot-testing of a Self-assessment tool for the implementation of the European Standards of Care for Newborn Health (ESCNH).

BMJ paediatrics open·2025

Related Experiment Video

Updated: May 10, 2026

Modeling Encephalopathy of Prematurity Using Prenatal Hypoxia-ischemia with Intra-amniotic Lipopolysaccharide in Rats
07:36

Modeling Encephalopathy of Prematurity Using Prenatal Hypoxia-ischemia with Intra-amniotic Lipopolysaccharide in Rats

Published on: November 20, 2015

Fetal brain genomic reprogramming following asphyctic preconditioning.

Kimberly E M Cox-Limpens, Johan S H Vles, Jana Schlechter

    BMC Neuroscience
    |June 27, 2013
    PubMed
    Summary

    Fetal asphyctic preconditioning protects the fetal brain by altering gene expression. This study reveals down-regulated neurotransmission and ion transport, and up-regulated epigenetic mechanisms, offering insights into neuroprotection strategies.

    More Related Videos

    Assessment of Ultrastructural Neuroplasticity Parameters After In Utero Transduction of the Developing Mouse Brain and Spinal Cord
    10:28

    Assessment of Ultrastructural Neuroplasticity Parameters After In Utero Transduction of the Developing Mouse Brain and Spinal Cord

    Published on: February 26, 2019

    A Novel Strategy Combining Array-CGH, Whole-exome Sequencing and In Utero Electroporation in Rodents to Identify Causative Genes for Brain Malformations
    08:22

    A Novel Strategy Combining Array-CGH, Whole-exome Sequencing and In Utero Electroporation in Rodents to Identify Causative Genes for Brain Malformations

    Published on: December 1, 2017

    Related Experiment Videos

    Last Updated: May 10, 2026

    Modeling Encephalopathy of Prematurity Using Prenatal Hypoxia-ischemia with Intra-amniotic Lipopolysaccharide in Rats
    07:36

    Modeling Encephalopathy of Prematurity Using Prenatal Hypoxia-ischemia with Intra-amniotic Lipopolysaccharide in Rats

    Published on: November 20, 2015

    Assessment of Ultrastructural Neuroplasticity Parameters After In Utero Transduction of the Developing Mouse Brain and Spinal Cord
    10:28

    Assessment of Ultrastructural Neuroplasticity Parameters After In Utero Transduction of the Developing Mouse Brain and Spinal Cord

    Published on: February 26, 2019

    A Novel Strategy Combining Array-CGH, Whole-exome Sequencing and In Utero Electroporation in Rodents to Identify Causative Genes for Brain Malformations
    08:22

    A Novel Strategy Combining Array-CGH, Whole-exome Sequencing and In Utero Electroporation in Rodents to Identify Causative Genes for Brain Malformations

    Published on: December 1, 2017

    Area of Science:

    • Neuroscience
    • Genomics
    • Developmental Biology

    Background:

    • Fetal asphyctic (FA) preconditioning mitigates brain damage from perinatal asphyxia.
    • Understanding FA's endogenous neuroprotection mechanisms is crucial for clinical applications in neonates.
    • Genomic reprogramming is a suspected contributor to FA's protective effects.

    Purpose of the Study:

    • To investigate whole-genome differential gene expression in the preconditioned rat fetal brain.
    • To identify specific genes and pathways affected by FA preconditioning.
    • To explore the role of genomic reprogramming in FA-induced neuroprotection.

    Main Methods:

    • FA preconditioning induced by reversible uterine circulation clamping on embryonic day 17.
    • Whole-genome transcription analysis using Affymetrix Gene1.0ST chips.
    • Differential gene expression analysis with Bioconductor Limma package and RT-qPCR validation.
    • Gene Set Enrichment Analysis (GSEA) to identify affected biological pathways.

    Main Results:

    • 53 transcripts were down-regulated and 35 were up-regulated in the FA group.
    • Down-regulated gene sets were primarily involved in neurotransmission and ion transport.
    • Up-regulated gene sets were mainly associated with nucleosomal structure and transcription, including Mecp2.

    Conclusions:

    • This study is the first to identify differential gene expression in fetal brain tissue following asphyctic preconditioning.
    • Down-regulation of neurotransmission and ion transport may limit energy consumption, preventing neuronal damage.
    • Up-regulated genes involved in nuclear functions suggest epigenetic mechanisms contribute to neuroprotection.