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A54145, a new lipopeptide antibiotic complex: isolation and characterization.
D S Fukuda1, R H Du Bus, P J Baker
1Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46285.
The Journal of Antibiotics
|June 1, 1990
Summary
Streptomyces fradiae produces the A54145 antibiotic complex, active against Gram-positive bacteria. Researchers purified and characterized eight antibacterial factors, detailing their molecular formulas and acyl side chains.
Area of Science:
- Microbiology and Natural Products Chemistry
Background:
- The A54145 complex is an acidic lipopeptide antibiotic produced by Streptomyces fradiae.
- This complex exhibits activity against Gram-positive bacteria.
Purpose of the Study:
- To isolate and purify individual antibacterial factors from the A54145 complex.
- To determine the molecular formulas and acyl side chain identities of these purified factors.
Main Methods:
- Isolation of the A54145 complex using Diaion HP-20 resin and Amberlite IRA-68 anion exchange resin.
- Purification of antibacterial factors A, A1, B, B1, C, D, E, and F via preparative reverse-phase high-performance liquid chromatography (HPLC) on C8 and C18 bonded-phase supports.
- Determination of molecular formulas and acyl side chain structures.
Main Results:
- Eight distinct antibacterial factors (A, A1, B, B1, C, D, E, F) were successfully purified.
- Molecular formulas were assigned to each factor, with variations in elemental composition noted.
- Acyl side chains were identified as 8-methylnonanoyl, n-decanoyl, and 8-methyldecanoyl, linked to specific factors.
Conclusions:
- The A54145 complex comprises multiple lipopeptide variants with distinct molecular structures.
- The characterization provides a foundation for understanding the structure-activity relationships of these novel antibiotics.