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Real-time Imaging of Leukotriene B4 Mediated Cell Migration and BLT1 Interactions with β-arrestin
Published on: December 23, 2010
[Leukotriene D4 activates BV2 microglia in vitro].
Zhuang Zhang1, Jiangyun Luo, Jing Huang
1Department of Pharmacology, Zhejiang University School of Medicine, Key Laboratory of Medical Neurobiology of Ministry of Health, Hangzhou 310058, China.
Summary
Leukotriene D4 (LTD4) activates microglia cells, increasing phagocytosis and IL-6 mRNA expression. CysLT1 receptor mediates IL-6, while CysLT2 receptor may negatively regulate LTD4-induced phagocytosis.
Area of Science:
- Neuroimmunology
- Cellular Biology
- Pharmacology
Background:
- Microglia play crucial roles in neuroinflammation.
- Cysteinyl leukotriene (CysLT) receptors are implicated in inflammatory responses.
Purpose of the Study:
- To investigate the effects of LTD4 and its receptor antagonists on BV2 microglia activation.
- To elucidate the roles of CysLT1 and CysLT2 receptors in LTD4-mediated microglial responses.
Main Methods:
- BV2 cells were treated with LTD4 and selective CysLT receptor antagonists (montelukast, HAMI 3379, BAY u9773).
- Assays included Western blotting, immunostaining, MTT reduction, fluorescent bead tracking, and RT-PCR.
- Protein expression, cell viability, phagocytosis, and IL-6 mRNA levels were analyzed.
Main Results:
- LTD4 enhanced phagocytosis and IL-6 mRNA expression in BV2 cells.
- LTD4-induced IL-6 mRNA expression was mediated by the CysLT1 receptor.
- Phagocytosis was further increased by CysLT2 antagonist HAMI 3379 and dual antagonist BAY u9773.
- Montelukast and BAY u9773 reduced LTD4-induced IL-6 mRNA expression.
Conclusions:
- LTD4 activates BV2 microglia, upregulating IL-6 via the CysLT1 receptor.
- CysLT2 receptor may exert negative regulation on LTD4-induced phagocytosis in microglia.
