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Published on: March 20, 2021
[Evaluation of methods for collection of peripheral blood stem cells in children]
Guomei Yin1, Zhuolan Shen, Fei Qin
1The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.
Insights
This study evaluated methods for collecting peripheral blood stem cells (PBSC) in children. Optimal PBSC collection requires choosing methods based on patient weight, total blood volume, and mobilization factors.
Area of Science:
- Pediatric Hematology
- Stem Cell Transplantation
- Cellular Therapy
Background:
- Peripheral blood stem cell (PBSC) transplantation is a critical therapeutic modality for pediatric patients.
- Effective mobilization and collection strategies are essential for successful outcomes in pediatric PBSC transplantation.
Purpose of the Study:
- To assess and compare different methods for collecting peripheral blood stem cells (PBSC) in pediatric patients and donors.
- To identify key factors influencing the efficiency and quality of pediatric PBSC collection.
Main Methods:
- Peripheral blood stem cells were collected from 20 pediatric patients and 11 donors using G-CSF or GM-CSF for mobilization.
- Hematopoietic stem cells were collected using CS-3000 Plus, COM TEC, or COBE Spectra apheresis devices.
- Specific protocols, including red blood cell pre-injection and low flow rates, were employed for children under 20 kg or with low hematocrit/blood volume.
Main Results:
- Successful PBSC collection was achieved in all 31 cases using the specified apheresis devices, with 1-5 apheresis sessions.
- The average yield was (7.9 ±2.9) x 10^6 CD34(+) cells/kg and (7.4 ±3.1) x 10^8 mononuclear cells/kg.
- A significant correlation was observed between the total CD34(+) cell count and pre-apheresis mononuclear cell counts and processed blood volume.
Conclusions:
- The choice of apheresis method for pediatric PBSC collection should be individualized.
- Factors such as patient body weight, total blood volume, and mobilization status are crucial for optimizing PBSC collection.
- Tailoring collection strategies ensures the procurement of high-quality PBSCs for pediatric transplantation.
Objective:
To evaluate the methods for collection of peripheral blood stem cells (PBSC) in children.
Methods:
Peripheral blood stem cells were collected from 20 child patients and 11 donors. The patients treated with chemotherapy, received G-CSF or GM-CSF and the donors received G-CSF for mobilization. When the peripheral blood (PB) leukocyte count reached to 5 X10(9)/L,the hematopoietic stem cells were collected with CS-3000 Plus, COM TEC or COBE Spectra blood cell separators from patients and donors. For children whose weight <20 kg,HCT <24% or TBV <1 100-1 650 ml,blood cell separators were pre-injected with the same type RBCs irradiated by 25 Gy of gamma-ray and with low flow rate (10-30 ml/min). The number of CD34(+) cell was detected by flow cytometry. The relationship of number of CD34(+) cell with mononuclear cell (MNC) and processed blood volume was analyzed.
Results:
Successful collection of the PBSCs with the CS- 3000 Plus (n=10), the COM TEC (n=3) and the COBE Spectra (n=18) was achieved in all the 31 cases with 1-5 aphereses used. Number of CD34(+) cells was (7.9 ±2.9) X 10(6)/kg and that of MNCs was (7.4 ±3.1) X 10(8)/kg. The total CD34(+) cell count was correlated with MNCs before aphaeresis and processed blood volume.
Conclusion:
For collection of high quality PBSCs, the appropriate methods should be chosen according to the body weight, TBV, mobilization of child patients/donors.
