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Determination of mephenesin in plasma by high-performance liquid chromatography with fluorimetric detection
P Guinebault1, C Colafranceschi, G Bianchetti
1Synthélabo-Recherche (L.E.R.S.), Meudon-la-Fôret, France.
Journal of Chromatography
|May 16, 1990
Abstract:
A procedure for the determination of the CNS-active muscle-relaxant mephenesin in plasma samples is described. The method involved a single-step extraction with diethyl ether followed by high-performance liquid chromatography separation and fluorimetric detection. Linearity of the detection response was observed between 1 ng/ml, which is the limit of determination, and 500 ng/ml. Inter-day assays, including quality-control samples, performed over a 4-month period, showed the method to be reproducible and precise, and suitable for routine analysis in pharmacokinetic studies.