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Updated: May 10, 2026
Impedance-based Real-time Measurement of Cancer Cell Migration and Invasion
Published on: April 2, 2020
Inhibitory effects of syk transfection on lung cancer cell invasion
Chuan-Liang Peng1, Ying Zhang, Qi-Feng Sun
1Department of Thoracic, The Second Hospital of Shandong University, Jinan, China. pechuliang@yahoo.com.cn
Objective:
Spleen tyrosine kinase (Syk) is closely related to tumor invasion and metastasis, and has been shown to have potential inhibitory effects in tumors. In this study, we constructed a eukaryotic expression vector for Syk and analyzed its effects on invasive ability of the A549 non-small cell lung cancer cell line in vitro.
Methods:
A fragment of Syk was obtained by RT-PCR from human lung cancer cells and cloned into the expression vector pLNCXSyk. After restriction endonuclease digestion, PCR and DNA sequencing confirmation, the recombinant Syk expression plasmid was transfected into A549 human lung cancer cells using lipofectamine protocols. After selection, the cells stably expressed Syk. Detection of Syk expression of the cells by RT-PCR, and invasive ability were examined.
Results:
The eukaryotic expression plamid pLNCXSyk was constructed and expressed stably in the A549 human lung cancer cells. The RT-PCR results showed that Syk mRNA expression was upregulated significantly (P<0.05). Lower invasion through a basal membrane were apparent after transfection (P<0.05).
Conclusions:
A eukaryotic expression plasmid to cause Syk expression in lung cancer cells can obviously inhibit their invasive ability in vitro.
Insights
Spleen tyrosine kinase (Syk) expression was successfully upregulated in lung cancer cells using a novel eukaryotic expression vector. This significantly inhibited the invasive capabilities of these cancer cells in vitro.
Area of Science:
- Molecular Biology
- Oncology
- Cancer Research
Background:
- Spleen tyrosine kinase (Syk) plays a crucial role in tumor invasion and metastasis.
- Syk has demonstrated potential as an inhibitory agent in various tumor types.
- Understanding Syk's role is vital for developing novel lung cancer therapies.
Purpose of the Study:
- To construct a eukaryotic expression vector for Syk.
- To analyze the effects of Syk on the invasive ability of A549 non-small cell lung cancer cells in vitro.
Main Methods:
- Obtained Syk fragment via RT-PCR from human lung cancer cells.
- Cloned Syk into the pLNCXSyk expression vector.
- Transfected A549 cells with the recombinant plasmid and confirmed stable Syk expression using RT-PCR.
Main Results:
- Successfully constructed and stably expressed the pLNCXSyk plasmid in A549 cells.
- Demonstrated significant upregulation of Syk mRNA expression (P<0.05).
- Observed a significant reduction in cell invasion through a basal membrane (P<0.05).
Conclusions:
- Eukaryotic expression of Syk in lung cancer cells effectively inhibits their invasive potential in vitro.
- Syk represents a promising therapeutic target for reducing lung cancer cell invasion.
