Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Solid-surface room-temperature phosphorescence detection for high-performance liquid chromatography.

A D Campiglia1, A Berthod, J D Winefordner

  • 1Department of Chemistry, University of Florida, Gainesville 32611.

Journal of Chromatography
|May 25, 1990
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Phosphorimetry.

Journal of research of the National Bureau of Standards. Section A, Physics and chemistry·2021
Same author

Photoluminescence spectroscopy of anthrathiophenes and benzonaphthothiophenes in Shpol'skii matrixes.

Talanta·2019
Same author

Recent advances on ionic liquid uses in separation techniques.

Journal of chromatography. A·2017
Same author

On the use of ionic liquids as mobile phase additives in high-performance liquid chromatography. A review.

Analytica chimica acta·2015
Same author

Revisiting resolution in hydrodynamic countercurrent chromatography: tubing bore effect.

Journal of chromatography. A·2015
Same author

Limonene in Arizona liquid systems used in countercurrent chromatography. II Polarity and stationary-phase retention.

Analytical and bioanalytical chemistry·2014
Same journal

Rapid synthesis of isoprenoid diphosphates and their isolation in one step using either thin layer or flash chromatography.

Journal of chromatography·2013
Same journal

[Electrophoresis of radionuclides on cellogel].

Journal of chromatography·2012
Same journal

Frontal affinity chromatography: theory for its application to studies on specific interactions of biomolecules.

Journal of chromatography·2010
Same journal

Cumulative author, subject, compound and special issue indexes. Volumes 601-650 (1992-1993).

Journal of chromatography·1994
Same journal

Determination of the calcium antagonist SIM6080 in plasma by high-resolution gas chromatography.

Journal of chromatography·1994
Same journal

Processing of envelope polypeptides of herpes simplex virus type 1. Demonstration of variation in different cell lines by high-performance liquid chromatography and radioimmunoprecipitation.

Journal of chromatography·1994
See all related articles

We optimized a two-nebulizer system for solid-surface room-temperature phosphorescence (SSRTP) detection in high-performance liquid chromatography (HPLC). This selective SSRTP detector offers sensitive, permanent records for analyzing phosphorescent compounds.

Area of Science:

  • Analytical Chemistry
  • Chromatography

Background:

  • High-performance liquid chromatography (HPLC) is a key analytical technique.
  • Developing selective and sensitive detectors enhances HPLC capabilities.
  • Solid-surface room-temperature phosphorescence (SSRTP) offers potential for sensitive detection.

Purpose of the Study:

  • To optimize a two-nebulizer system for SSRTP detection in HPLC.
  • To evaluate SSRTP as a selective, permanent record detector.
  • To compare SSRTP detection with traditional ultraviolet detection.

Main Methods:

  • Optimization of a two-nebulizer system for SSRTP.
  • Application of SSRTP detection to HPLC analysis.
  • Comparison of chromatographic parameters and analytical figures of merit.

Related Experiment Videos

  • Analysis of five well-known phosphorescent compounds.

    • Main Results:

    • Achieved satisfactory linear dynamic ranges and low limits of detection (nanogram and subnanogram levels).
    • Demonstrated the feasibility of the SSRTP detector for HPLC.
    • Showcased the selectivity of SSRTP for identifying overlapped compounds in complex mixtures.
    • Confirmed SSRTP as a valuable tool for incomplete chromatographic separations.

    Conclusions:

    • The optimized two-nebulizer SSRTP system is a feasible and sensitive detector for HPLC.
    • SSRTP provides a selective and permanent record, advantageous for complex sample analysis.
    • This method enhances the analytical capabilities of HPLC for phosphorescent compounds.