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Related Experiment Videos

Staphylococcal slime: a cautionary tale.

D T Drewry1, L Galbraith, B J Wilkinson

  • 1School of Chemistry, University of Hull, England.

Journal of Clinical Microbiology
|June 1, 1990
PubMed
Summary
This summary is machine-generated.

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Researchers found that "slime" from Staphylococcus epidermidis grown in broth-agar medium was mainly an artifact of the agar. Further studies on bacterial slime should avoid agar and use defined media.

Area of Science:

  • Microbiology
  • Biomaterials Science

Background:

  • Slime production by Staphylococcus epidermidis is implicated in biomedical device colonization.
  • The immune system effects of bacterial slime are under investigation.

Purpose of the Study:

  • To isolate, purify, and chemically characterize slime produced by S. epidermidis.
  • To determine the origin and composition of crude slime preparations.

Main Methods:

  • Cultivation of S. epidermidis on tryptic soy broth-agar medium.
  • Chemical characterization of isolated slime using gas-liquid chromatography and mass spectrometry.
  • Analysis of methylation residues.

Main Results:

  • Crude slime preparations from S. epidermidis and controls showed high galactose content.

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  • Analysis indicated that agar in the growth medium was the primary source of the crude slime.
  • Preparation procedures likely resulted in slime characterization as an artifact.

    • Conclusions:

    • The commonly observed high-galactose slime is likely an artifact of using agar-based media.
    • Solid surface growth may be crucial for actual bacterial slime production.
    • Future research requires chemically defined media and avoidance of agar to accurately study bacterial slime.