Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Imaging Studies II: Positron Emission Tomography and Scintigraphy01:25

Imaging Studies II: Positron Emission Tomography and Scintigraphy

Positron Emission Tomography (PET) is a medical imaging technique that provides crucial insights into the body's physiological functions at a molecular level. It is an indispensable resource for diagnosing, staging, and monitoring various illnesses, notably cancer, neurological disorders, and cardiovascular conditions.
Fundamental Principles of PET
Immunocytochemistry and Immunohistochemistry01:22

Immunocytochemistry and Immunohistochemistry

Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
These...
Imaging Studies for Cardiovascular System IV: CMRI01:21

Imaging Studies for Cardiovascular System IV: CMRI

Cardiovascular magnetic resonance imaging, or CMRI, is a non-invasive diagnostic test that employs a magnetic field and radiofrequency waves to create precise images of the heart and arteries. It provides comprehensive information about cardiac anatomy, function, perfusion, and tissue characterization without ionizing radiation.IndicationsCMRI diagnoses various heart conditions, including tissue damage from heart attacks, ischemic heart disease, myocarditis, aortic issues (tears, aneurysms,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correction: Discovery and optimization of tau targeted protein degraders enabled by patient induced pluripotent stem cells-derived neuronal models of tauopathy.

Frontiers in cellular neuroscience·2026
Same author

A repeat expansion in GOLGA8A is a major risk factor for atypical frontotemporal lobar degeneration with ubiquitin-positive inclusions.

Nature genetics·2026
Same author

Efficient In Vivo Pharmacological Inhibition of ΔFOSB, an AP-1 Transcription Factor, in the Brain.

ACS chemical neuroscience·2026
Same author

Environmental impact, cost, and acceptability of a laboratory sustainability certification program for biomedical research in an academic medical center.

The journal of climate change and health·2026
Same author

Dopaminergic mechanisms supporting hippocampal postencoding dynamics in humans.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Discovery of small molecules and a druggable groove that regulate DNA binding and release of the AP-1 transcription factor ΔFOSB.

The Journal of biological chemistry·2025

Related Experiment Video

Updated: May 10, 2026

Autoradiography as a Simple and Powerful Method for Visualization and Characterization of Pharmacological Targets
10:16

Autoradiography as a Simple and Powerful Method for Visualization and Characterization of Pharmacological Targets

Published on: March 12, 2019

Class I HDAC imaging using [ (3)H]CI-994 autoradiography.

Yajie Wang1, Yan-Ling Zhang, Krista Hennig

  • 1Athinoula A. Martinos Center, Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA, USA.

Epigenetics
|June 28, 2013
PubMed
Summary

Researchers developed a new radioactive tracer, [3H]CI-994, to visualize class I histone deacetylases (HDACs) in the brain. This tool maps HDAC1-3 distribution and density, aiding epigenetic regulation studies in diseases.

Keywords:
CI-994HDACautradiographybenzamideclass Idensityinhibitor

More Related Videos

Enhancing Efficiency and Radiolabeling Yields of Carbon-11 Radioligands for Clinical Research Using the Loop Method
09:08

Enhancing Efficiency and Radiolabeling Yields of Carbon-11 Radioligands for Clinical Research Using the Loop Method

Published on: December 20, 2024

Receptor Autoradiography Protocol for the Localized Visualization of Angiotensin II Receptors
12:03

Receptor Autoradiography Protocol for the Localized Visualization of Angiotensin II Receptors

Published on: June 7, 2016

Related Experiment Videos

Last Updated: May 10, 2026

Autoradiography as a Simple and Powerful Method for Visualization and Characterization of Pharmacological Targets
10:16

Autoradiography as a Simple and Powerful Method for Visualization and Characterization of Pharmacological Targets

Published on: March 12, 2019

Enhancing Efficiency and Radiolabeling Yields of Carbon-11 Radioligands for Clinical Research Using the Loop Method
09:08

Enhancing Efficiency and Radiolabeling Yields of Carbon-11 Radioligands for Clinical Research Using the Loop Method

Published on: December 20, 2024

Receptor Autoradiography Protocol for the Localized Visualization of Angiotensin II Receptors
12:03

Receptor Autoradiography Protocol for the Localized Visualization of Angiotensin II Receptors

Published on: June 7, 2016

Area of Science:

  • Neuroscience
  • Biochemistry
  • Pharmacology

Background:

  • Histone deacetylases (HDACs) are crucial epigenetic regulators.
  • Class I HDACs (isoforms 1-3) play significant roles in cellular processes.
  • Developing specific probes for HDACs is essential for understanding their function and developing therapies.

Purpose of the Study:

  • To develop and validate [3H]CI-994 as an autoradiography probe for ex vivo imaging of class I HDACs in the rodent brain.
  • To characterize the binding kinetics and distribution of class I HDACs using [3H]CI-994.
  • To correlate autoradiographic findings with protein expression levels.

Main Methods:

  • Synthesis and characterization of the radioactive tracer [3H]CI-994.
  • Optimization of autoradiography protocols for ex vivo brain tissue.
  • Saturation and kinetic binding experiments to determine Kd and Bmax values.
  • Competition assays with known HDAC inhibitors.
  • Western blot analysis for semi-quantitative HDAC isoform measurement.

Main Results:

  • [3H]CI-994 demonstrated high specific binding (up to 80%) for class I HDACs.
  • Slow binding kinetics were observed for [3H]CI-994 with isolated enzymes and ex vivo.
  • Regional distribution revealed highest HDAC1-3 density in the cerebellum, hippocampus, and cortex.
  • Kd values ranged from 9.17 to 15.6 nM, with a whole-brain Bmax of 12.9 pmol·mg(-1) protein.
  • Autoradiographic data correlated well with western blot analysis, indicating representation of HDAC1-3 protein density.

Conclusions:

  • [3H]CI-994 is the first autoradiography tool developed for imaging class I HDAC enzymes.
  • This probe enables detailed mapping of HDAC1-3 distribution and density in the CNS.
  • The tool has potential applications beyond the CNS for evaluating HDAC inhibition in novel therapies and studying disease-related epigenetic regulation.