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A radioimmunoassay for antibodies to the Ro/SSA particle
1Department of Medicine, Oklahoma University College of Medicine, Oklahoma Health Sciences Center, Oklahoma City.
Journal of Immunological Methods
|July 20, 1990
Abstract:
A radioimmunoassay (RIA) for antibodies to the Ro/SSA particle is described using Iodogen to radiolabel the antigenic protein moiety of Ro/SSA with 125I. RIA methods utilizing either 33% saturated ammonium sulfate (NH4)2SO4 or 3.5% polyethylene glycol to separate bound from free antigen are comparable. Either method is similar to ELISA in sensitivity and specificity. This RIA provides a fluid phase assay for the Ro/SSA-anti-Ro/SSA system not previously available.