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Related Concept Videos

T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...

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A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes
07:59

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Published on: March 25, 2014

Proteins as T cell antigens: methods for high-throughput identification.

Daniel Grubaugh1, Jessica Baker Flechtner, Darren E Higgins

  • 1Department of Microbiology and Immunobiology, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115-5701, United States.

Vaccine
|June 29, 2013
PubMed
Summary

Developing new vaccines requires identifying T cell antigens, not just antibody targets. Advances in high-throughput screening are crucial for creating effective vaccines against challenging diseases.

Keywords:
High-throughput screeningProtein antigenReverse vaccinology, MHCSubunit vaccineT cells

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Area of Science:

  • Immunology
  • Vaccinology
  • Proteomics

Background:

  • Vaccines are vital for preventing infectious diseases and treating chronic conditions like cancer.
  • Current vaccines primarily rely on antibody responses, which are insufficient for many diseases.
  • T cell responses are increasingly recognized as critical for comprehensive immunity.

Purpose of the Study:

  • To review advances in high-throughput identification of T cell antigens.
  • To highlight the need for improved methods in T cell antigen discovery.
  • To discuss the development of novel and effective vaccines.

Main Methods:

  • Genome/proteome-wide screening for vaccine targets.
  • High-throughput proteomic screening approaches.
  • Development of methods for identifying T cell targets across diverse populations.

Main Results:

  • Significant progress has been made in identifying T cell antigens.
  • Challenges remain in applying high-throughput screening for T cell targets.
  • New methods are emerging for broad T cell immunity identification.

Conclusions:

  • High-throughput T cell antigen identification is essential for next-generation vaccines.
  • Overcoming current limitations will enable broader vaccine development.
  • Advances pave the way for more efficacious vaccines against various diseases.