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Updated: May 10, 2026

Aseptic Laboratory Techniques: Volume Transfers with Serological Pipettes and Micropipettors
Published on: May 31, 2012
Implementing AORN recommended practices for sterile technique
1Women's Services, Minimally Invasive Gynecology, and Palliative Care at Inova Fair Oaks Hospital, Fairfax, VA, USA.
Abstract:
Using sterile technique helps prevent the surgical environment from becoming contaminated and thus can help reduce the incidence of surgical site infection. The AORN "Recommended practices for sterile technique" provides guidance for setting up, maintaining, and monitoring a sterile field. Topics include the use of surgical attire and personal protective equipment; appropriate selection and evaluation of surgical gowns, gloves, and drape products for each procedure; use of sterile technique to don sterile gowns and gloves; appropriate methods for establishing and monitoring a sterile field; and techniques to ensure that items such as surgical instruments that may be contaminated are not used. Breaks in sterile technique should be corrected immediately unless the actions necessary would endanger the patient. If remedial actions must be delayed, they should be undertaken as soon as possible. Adhering to best practices for sterile technique requires remaining up to date with new approaches and incorporating these into quality initiatives.
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