Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

3D Expansion-PALM (PhotoActivated Localization Microscopy) Dissects Protein-Protein Interactions Down to the Molecular Scale in Bacteria.

Microorganisms·2026
Same author

Testosterone protects from metabolic syndrome-associated lung dysfunction in a high-fat diet rabbit model.

Endocrinology·2026
Same author

OPA1 Deficiency Impairs NGF Signaling and Drives Sympathetic Neurodegeneration.

JACC. Basic to translational science·2026
Same author

Editorial: Novel approaches to foster brain plasticity in neurodevelopmental and neurodegenerative disorders.

Frontiers in cellular neuroscience·2026
Same author

Mechanical de-skewing enables high-resolution imaging of thin tissue slices with a mesoSPIM light-sheet microscope.

Biomedical optics express·2026
Same author

Ultrasensitive Saliva-Based Detection of Early Alzheimer's Disease Biomarkers via Nanoparticle-Enhanced Evanescent Scattering Microscopy.

ACS sensors·2026
Same journal

EC-isHCR: A rapid method for in situ hybridization chain reaction in diverse animal samples.

Methods (San Diego, Calif.)·2026
Same journal

Single-Molecule methods to investigate mechanisms of transcription by RNA polymerase of Mycobacterium tuberculosis.

Methods (San Diego, Calif.)·2026
Same journal

Detection and sequencing of Usutu virus during mosquito surveillance: Use of multiple assays and techniques for identification at low levels.

Methods (San Diego, Calif.)·2026
Same journal

Experimental validation of an AI-driven digital healthcare platform for oral health behavior and plaque assessment among vietnamese children.

Methods (San Diego, Calif.)·2026
Same journal

Zeta potential: An efficient and cost-effective alternative for investigating cell-surface interactions.

Methods (San Diego, Calif.)·2026
Same journal

An automated workflow for quantifying the formation of synuclein aggregates in human dopaminergic neurons.

Methods (San Diego, Calif.)·2026
See all related articles

Related Experiment Video

Updated: May 10, 2026

Light Sheet-based Fluorescence Microscopy of Living or Fixed and Stained Tribolium castaneum Embryos
10:15

Light Sheet-based Fluorescence Microscopy of Living or Fixed and Stained Tribolium castaneum Embryos

Published on: April 28, 2017

Correlative two-photon and light sheet microscopy.

Ludovico Silvestri1, Anna Letizia Allegra Mascaro1, Irene Costantini1

  • 1European Laboratory for Non-Linear Spectroscopy, University of Florence, Italy.

Methods (San Diego, Calif.)
|June 29, 2013
PubMed
Summary
This summary is machine-generated.

This study presents a novel correlative framework integrating in vivo two-photon imaging and ex vivo light sheet microscopy. This method allows detailed visualization and 3D anatomical contextualization of individual neurons within the central nervous system.

Keywords:
Brain imagingCorrelative microscopyLight sheet microscopyTwo-photon fluorescence microscopy

More Related Videos

Light-sheet Fluorescence Microscopy for the Study of the Murine Heart
08:42

Light-sheet Fluorescence Microscopy for the Study of the Murine Heart

Published on: September 15, 2018

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions
13:43

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions

Published on: June 24, 2013

Related Experiment Videos

Last Updated: May 10, 2026

Light Sheet-based Fluorescence Microscopy of Living or Fixed and Stained Tribolium castaneum Embryos
10:15

Light Sheet-based Fluorescence Microscopy of Living or Fixed and Stained Tribolium castaneum Embryos

Published on: April 28, 2017

Light-sheet Fluorescence Microscopy for the Study of the Murine Heart
08:42

Light-sheet Fluorescence Microscopy for the Study of the Murine Heart

Published on: September 15, 2018

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions
13:43

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions

Published on: June 24, 2013

Area of Science:

  • Neuroscience
  • Microscopy
  • Computational Biology

Background:

  • Central nervous system information processing occurs across multiple spatial and temporal scales.
  • Individual imaging techniques offer limited views of brain functionality.
  • Integrating complementary methods is crucial for a comprehensive understanding of brain activity.

Purpose of the Study:

  • To develop and demonstrate a correlative framework for integrating in vivo and ex vivo imaging data.
  • To enable the contextualization of high-resolution in vivo neuronal dynamics within a large-scale 3D brain anatomy.
  • To establish a method for segmenting entire neurons from combined imaging datasets.

Main Methods:

  • Correlative framework integrating in vivo two-photon fluorescence imaging and ex vivo light sheet microscopy.
  • Utilizing blood vessels as a reference chart for data alignment.
  • Imaging of apical dendritic arbor of cortical pyramidal neurons in living thy1-GFP-M mice.
  • Large-scale brain reconstruction using light sheet microscopy.

Main Results:

  • Successful integration of data from two-photon and light sheet microscopy.
  • Identification of in vivo imaged neuronal structures within the ex vivo large-scale brain reconstruction.
  • Demonstration of whole pyramidal neuron segmentation starting from apical portions.
  • Contextualization of neuron dynamics within a 3D anatomical framework.

Conclusions:

  • The presented correlative approach effectively integrates multi-scale brain imaging data.
  • This method provides a powerful tool for understanding neuronal function in its anatomical context.
  • Future research can leverage this framework for detailed neuroanatomical and functional studies.