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Distribution of cytomegalovirus gN variants and associated clinical sequelae in infants

Edyta Paradowska1, Agnieszka Jabłońska, Mirosława Studzińska

  • 1Laboratory of Molecular Virology and Biological Chemistry, Institute of Medical Biology, Polish Academy of Sciences, Lodz, Poland. eparadowska@cbm.pan.pl

Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology
|June 29, 2013
PubMed
Abstract

Insights

Human cytomegalovirus (HCMV) gN genotypes may indicate infection severity in children. Specific genotypes like gN4 are linked to neurological issues, suggesting gN typing as a marker for symptomatic HCMV infection.

Area of Science:

  • Virology
  • Pediatric Infectious Diseases
  • Genetics

Background:

  • Human cytomegalovirus (HCMV) is a leading cause of congenital infections.
  • The impact of viral strains and loads on HCMV infection outcomes remains debated.

Purpose of the Study:

  • To analyze gN gene variants in HCMV strains from infected children.
  • To correlate viral genotype, viral load, and clinical sequelae.

Main Methods:

  • Genotyping of HCMV strains using RFLP analysis of PCR-amplified fragments.
  • Quantification of viral load via real-time PCR.
  • Study included newborns and children with congenital or postnatal HCMV infection.

Main Results:

  • HCMV genotypes gN3b, gN4b, and gN4c were prevalent.
  • No significant difference in gN genotype distribution between congenital and postnatal infections.
  • HCMV gN4 genotype associated with increased risk of neurological disorders (p=0.045).
  • Higher viral loads observed in congenitally infected children.
  • gN2 and gN4 genotypes may indicate severe manifestations; gN1 and gN3b suggest less pathogenicity.

Conclusions:

  • HCMV gN genotype can serve as a virological marker for symptomatic HCMV infection in newborns.
  • gN genotyping may aid in predicting disease severity and guiding clinical management.
Keywords:
CICNSCSFCongenital infectionGEGlycoprotein NHCMVHuman cytomegalovirusIUGRORRFLPSNHLUPGMAcentral nervous systemcerebrospinal fluidconfidence intervalgCgNgenome equivalentsglycoprotein Nglycoprotein complexhuman cytomegalovirusintrauterine growth retardationnPCRnested polymerase chain reactionodds ratiorestriction fragment length polymorphismsensorineural hearing lossunweighted pair group method

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