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Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma
Published on: April 12, 2019
Bone morphogenetic protein 9 overexpression reduces osteosarcoma cell migration and invasion
Zilan Lv1, Dandan Yang, Jie Li
1Key Laboratory of Diagnostic Medicine, Chinese Ministry of Education, School of Clinical Diagnostic and Laboratory Medicine, Chongqing Medical University, Chongqing 400016, People's Republic of China.
Molecules and Cells
|June 29, 2013
Summary
Bone morphogenetic protein 9 (BMP9) inhibits osteosarcoma cell migration and invasion. This occurs via a Smad-dependent pathway, reducing matrix metalloproteinase 9 (MMP9) expression and activity.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- Transforming growth factor-β (TGF-β) family members, including Bone Morphogenetic Proteins (BMPs), are implicated in cancer progression.
- The specific role of Bone Morphogenetic Protein 9 (BMP9) in osteosarcoma (OS) remains unclear.
Purpose of the Study:
- To investigate the expression of BMP9 and its receptors in osteosarcoma cell lines.
- To determine the effect of BMP9 on the biological behaviors, including migration and invasion, of osteosarcoma cells.
Main Methods:
- Reverse transcription-polymerase chain reaction (RT-PCR) was used to analyze gene expression.
- BMP9 expression was manipulated (overexpression and silencing) in OS cell lines (143B and MG63).
- Analysis included assessment of Smad protein phosphorylation, ID1 expression, and matrix metalloproteinase 9 (MMP9) activity.
Main Results:
- BMP9 and its receptors are expressed in osteosarcoma cell lines.
- BMP9 overexpression inhibited in vitro cell migration and invasion.
- BMP9 increased Smad1/5/8 phosphorylation and ID1 expression, while decreasing MMP9 expression and activity.
Conclusions:
- BMP9 inhibits osteosarcoma cell migration and invasion.
- This inhibition is mediated through a Smad-dependent pathway.
- BMP9 downregulates MMP9 expression and activity, contributing to reduced invasiveness.
