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In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

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Doppler Optical Coherence Tomography of Retinal Circulation

Doppler Optical Coherence Tomography of Retinal Circulation

Published on: September 18, 2012

Microcirculation imaging based on full-range high-speed spectral domain correlation mapping optical coherence

Hrebesh M Subhash¹, Martin J Leahy

¹National University of Ireland, Tissue Optics and Microcirculation Imaging Facility, National Biophotonics and Imaging Platform, University Road, Galway, Ireland. hrebeshms@gmail.com

Journal of Biomedical Optics

|June 29, 2013

Summary

A new spectral-domain correlation mapping optical coherence tomography (cm-OCT) method provides clear, full-range microcirculation imaging. This technique enhances flow imaging sensitivity for deep tissues, improving disease research.

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Area of Science:

Biomedical Optics
Medical Imaging
Physiology

Background:

Microcirculation imaging is crucial for understanding disease pathophysiology in clinical and fundamental research.
Current imaging methods face limitations in speed, range, and sensitivity, particularly for deep tissues.

Purpose of the Study:

To present a full-range spectral-domain correlation mapping optical coherence tomography (cm-OCT) method for complex-conjugate-free microcirculation mapping.
To enhance acquisition speed and imaging depth for in vivo microcirculation studies.

Main Methods:

Developed a high-speed (91 kHz) spectrometer with a modified scanning protocol for cm-OCT.
Implemented a mirror image elimination technique using linear phase modulation and probe beam offset.
Utilized a Hilbert transform-based algorithm in conjunction with cm-OCT for complex-conjugate-free, full-range imaging.

Main Results:

Achieved high-speed, wide-scan-range cm-OCT imaging with artifact elimination.
Demonstrated an imaging sensitivity of ~105 dB near the zero-delay line with ~20 dB roll-off over ±3 mm depth.
Estimated axial and lateral resolutions of ~12 μm and ~30 μm, respectively.

Conclusions:

The proposed cm-OCT method effectively eliminates complex conjugate artifacts, enabling full-range microcirculation imaging.
Enhanced flow imaging sensitivity, especially for deep tissues, by utilizing the zero-delay line and doubling the imaging range.
This advancement offers significant potential for improved diagnostic and research capabilities in microcirculation studies.