Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 10, 2026

Decellularization of Whole Human Heart Inside a Pressurized Pouch in an Inverted Orientation
06:28

Decellularization of Whole Human Heart Inside a Pressurized Pouch in an Inverted Orientation

Published on: November 26, 2018

Decellularized whole heart for bioartificial heart.

Hug Aubin1, Alexander Kranz, Jörn Hülsmann

  • 1Department for Cardiovascular Surgery, Heinrich Heine University, Duesseldorf, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|June 29, 2013
PubMed
Summary

Researchers developed a whole-heart decellularization method using automated perfusion. This creates native extracellular matrix (ECM) 3D templates preserving heart anatomy and vascular networks for future bioartificial heart research.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Anxiety, depression and health-related quality of life in patients with thoracic aortic disease: a longitudinal study in a cardiothoracic outpatient clinic.

Frontiers in cardiovascular medicine·2026
Same author

Mid- to long-term results of minimally invasive repair of Barlow's disease with posterior leaflet resection and chordal transfer.

General thoracic and cardiovascular surgery·2026
Same author

Single-Center Experience with 15 VitalFlow ECMO Deployments for VA- and VV-ECMO Support: Deployment Characteristics, Outcomes, and Complications.

Journal of cardiovascular development and disease·2026
Same author

Preoperative MELD score variants and survival trajectories following in acute type A aortic dissection with follow‑up up to three years.

Journal of cardiothoracic surgery·2026
Same author

Purinergic signaling modulates endothelial-to-mesenchymal transition in human aortic valve endothelial cells.

BBA advances·2026
Same author

Validation of Aortic Blood Flow Simulations During Extracorporeal Circulation Using Phase Contrast Magnetic Resonance Imaging.

Artificial organs·2026

Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Tissue Engineering

Background:

  • Whole-organ decellularization yields 3D extracellular matrix (ECM) scaffolds.
  • Synthetic materials cannot replicate the complexity of natural ECM.
  • The native heart's intricate structure is crucial for its function.

Purpose of the Study:

  • To develop a whole-heart decellularization method.
  • To create native cardiac ECM 3D templates.
  • To preserve native heart anatomy and ECM characteristics.

Main Methods:

  • Software-controlled automated coronary perfusion.
  • Utilized standard decellularization detergents.
  • Focused on whole-organ decellularization of the heart.

More Related Videos

Procedure for Decellularization of Porcine Heart by Retrograde Coronary Perfusion
11:30

Procedure for Decellularization of Porcine Heart by Retrograde Coronary Perfusion

Published on: December 6, 2012

Neonatal Cardiac Scaffolds: Novel Matrices for Regenerative Studies
08:16

Neonatal Cardiac Scaffolds: Novel Matrices for Regenerative Studies

Published on: November 5, 2016

Related Experiment Videos

Last Updated: May 10, 2026

Decellularization of Whole Human Heart Inside a Pressurized Pouch in an Inverted Orientation
06:28

Decellularization of Whole Human Heart Inside a Pressurized Pouch in an Inverted Orientation

Published on: November 26, 2018

Procedure for Decellularization of Porcine Heart by Retrograde Coronary Perfusion
11:30

Procedure for Decellularization of Porcine Heart by Retrograde Coronary Perfusion

Published on: December 6, 2012

Neonatal Cardiac Scaffolds: Novel Matrices for Regenerative Studies
08:16

Neonatal Cardiac Scaffolds: Novel Matrices for Regenerative Studies

Published on: November 5, 2016

Main Results:

  • Successfully created native ECM-derived 3D cardiac templates.
  • Preserved basic cardiac anatomy and the vascular network.
  • Maintained critical ECM characteristics of the native heart.

Conclusions:

  • Automated perfusion enables reproducible whole-heart decellularization.
  • Cardiac ECM templates can advance understanding of cardiac biology.
  • This approach may facilitate the development of a bioartificial heart.