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Updated: May 10, 2026

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Aptamer-directed lanthanide chelate self-assembly for rapid thrombin detection.

Henna Päkkilä1, Sami Blom, Kari Kopra

  • 1Department of Biotechnology, University of Turku, Tykistökatu 6 A 6th floor, FI-20520 Turku, Finland. henna.pakkila@utu.fi

The Analyst
|June 29, 2013
PubMed
Summary

We developed a rapid and sensitive assay for detecting thrombin using lanthanide chelate complementation. This homogeneous method offers high specificity and eliminates autofluorescence for improved protein detection.

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Biotechnology

Background:

  • Homogeneous assays for protein detection often suffer from limitations due to autofluorescence.
  • Accurate and rapid detection of thrombin is crucial in various biomedical applications.

Purpose of the Study:

  • To develop a sensitive and rapid homogeneous assay for thrombin detection.
  • To utilize lanthanide chelate complementation for enhanced signal generation and specificity.

Main Methods:

  • Employing a lanthanide chelate complementation strategy with aptamer-based analyte binding.
  • Utilizing time-resolved fluorescence detection to minimize background noise.
  • Developing a homogeneous assay format for direct thrombin measurement.

Main Results:

  • Achieved sensitive detection of thrombin with high specificity.
  • Demonstrated a rapid assay format with maximum signal obtained within five minutes.
  • Successfully eliminated short-lifetime autofluorescence inherent in other homogeneous assays.

Conclusions:

  • Lanthanide chelate complementation provides a robust platform for sensitive and specific homogeneous protein detection.
  • The developed assay is rapid and overcomes limitations of traditional methods.
  • This approach is adaptable for detecting other proteins by utilizing specific binders to separate epitopes.