Related Experiment Video
Updated: May 10, 2026

5/6th Nephrectomy in Combination with High Salt Diet and Nitric Oxide Synthase Inhibition to Induce Chronic Kidney Disease in the Lewis Rat
Published on: July 3, 2013
High-protein diets and renal status in rats
V A Aparicio1, E Nebot, R García-del Moral
1Department of Physiology, School of Pharmacy, Faculty of Sport Sciences and Institute of Nutrition and Food Technology, University of Granada, Spain. virginiaparicio@ugr.es
Introduction:
High-protein (HP) diets might affect renal status. We aimed to examine the effects of a HP diet on plasma, urinary and morphological renal parameters in rats.
Material And Methods:
Twenty Wistar rats were randomly distributed in 2 experimental groups with HP or normal-protein (NP) diets over 12 weeks.
Results And Discussion:
Final body weight was a 10%lower in the HP group (p < 0.05) whereas we have not observed differences on food intake, carcass weight and muscle ashes content. No significant clear differences were observed on plasma parameters, whereas urinary citrate was an 88% lower in the HP group (p = 0.001) and urinary pH a 15% more acidic (p < 0.001). Kidney wet mass was ~22 heavier in the HP group (p < 0.001). Renal mesangium area was a 32% higher in the HP group (p < 0.01). Glomerular 1 and 2 were also ~30 higher in the HP diet (p < 0.01 and p < 0.05, respectively) and glomerular area a 13% higher (p < 0.01).
Conclusion:
High-protein diet promoted a worse renal profile, especially on urinary and morphological markers, which could increase the risk for developing renal diseases in the long time.
Related Concept Videos
Chronic Kidney Disease III: Interprofessional Care
Serum Studies: Renal Function Tests
Nephrotic Syndrome II : Assessment and Medical Management
Acute Kidney Injury V: Interprofessional Care
Renal Regulation of Acid-Base Balance
In the kidneys, cells within the proximal convoluted tubules (PCT) and the collecting ducts secrete hydrogen ions (H+) into the tubular fluid. Specifically, in the PCT, Na+/H+ antiporters secrete H+ while reabsorbing Na+.
However, the intercalated cells in...
Acute Kidney Injury IV: Diagnostic Studies and Prevention

