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Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems
Published on: September 23, 2021
A mass-differentiated library strategy for identification of sugar nucleotidyltransferase activities from cell
Kwang-Seuk Ko1, Rahman M Mizanur, Joy M Jackson
1Department of Chemistry, Iowa State University, Ames, IA 50011, USA.
Analytical Biochemistry
|July 2, 2013
Abstract:
Sugar nucleotidyltransferases, or nucleotide sugar pyrophosphorylases, are ubiquitous enzymes whose activities have been correlated to disease states and pathogen virulence. Here we report a rapid "one-pot" method to identify a range of sugar nucleotidyltransferase activities of purified proteins or in cell lysates using a mass-differentiated carbohydrate library designed for mass spectrometry-based analysis.
