Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Computer assisted efficiency testing of different sampling methods for selective nuclear graphic tablet morphometry.

J C Fleege1, P J van Diest, J P Baak

  • 1Institute of Pathology, Free University Hospital, Amsterdam, The Netherlands.

Laboratory Investigation; a Journal of Technical Methods and Pathology
|August 1, 1990
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Quantitative immunohistochemistry of estrogen and progesterone receptor positivity in ovarian tumors : a rapid and reliable stereological approach.

Methods in molecular medicine·2011
Same author

Potential clinical uses of laser scan microscopy.

Applied optics·2010
Same author

Keeping osteocytes alive: a comparison of drilling and hammering k-wires into bone.

The Journal of hand surgery, European volume·2008
Same author

mTOR in squamous cell carcinoma of the oesophagus: a potential target for molecular therapy?

Journal of clinical pathology·2008
Same author

HIF-1A gene mutations associated with higher microvessel density in endometrial carcinomas.

Histopathology·2008
Same author

Transient infection of freshly isolated human colorectal tumor cells by reovirus T3D intermediate subviral particles.

Cancer gene therapy·2008

The random and raster sampling methods are more efficient for nuclear morphometry, requiring smaller sample sizes for precise measurements compared to zone and convenience methods. This improves measurement reproducibility in pathological assessments.

Area of Science:

  • Pathology
  • Biomedical Engineering
  • Quantitative Biology

Background:

  • Nuclear morphometry is crucial for quantitative pathological assessments.
  • The precision of morphometric analysis depends heavily on the sampling strategy.
  • Efficient sampling minimizes effort while maximizing measurement accuracy.

Purpose of the Study:

  • To evaluate the efficiency of four random sampling methods: random, zone, at convenience, and raster.
  • To determine the optimal sampling strategy for nuclear morphometry in pathological studies.
  • To compare sample sizes required for achieving a 2.5% measurement precision.

Main Methods:

  • Computer-simulated sampling of 1000 nuclei from 12 study populations (endometrial hyperplasias and carcinomas).
  • Systematic measurement of nuclear features (area, perimeter, axes, shape factors) by an experienced morphometrist.

Related Experiment Videos

  • Comparative analysis of sample sizes needed for different sampling methods to reach a 2.5% precision level.

    • Main Results:

    • Random and raster sampling methods required significantly smaller sample sizes (max 200 nuclei for area) compared to zone and at convenience methods.
    • For features like perimeter and shape factors, even smaller sample sizes were sufficient with random and raster methods.
    • The efficiency difference is attributed to the ability of random and raster methods to sample across the entire field, accommodating spatial variations.

    Conclusions:

    • Random and raster sampling methods offer superior efficiency and reproducibility in nuclear morphometry.
    • These methods are better suited for handling clustered data or gradients in nuclear feature distribution.
    • Implementing random or raster sampling can enhance the reliability of quantitative pathological assessments.