Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Pleiotropy01:33

Pleiotropy

Pleiotropy is the phenomenon in which a single gene impacts multiple, seemingly unrelated phenotypic traits. For example, defects in the SOX10 gene cause Waardenburg Syndrome Type 4, or WS4, which can cause defects in pigmentation, hearing impairments, and an absence of intestinal contractions necessary for elimination. This diversity of phenotypes results from the expression pattern of SOX10 in early embryonic and fetal development. SOX10 is found in neural crest cells that form melanocytes,...
Source And Potency Of Stem Cells01:27

Source And Potency Of Stem Cells

Stem cells are undifferentiated cells with extensive self-renewal properties that help them maintain their population during the fetal and adult stages of life. They can specialize in all cell types of the human body. However, their differential potential may vary and can be classified into five types. Stem cells can be (1) Totipotent, (2) Pluripotent, (3) Multipotent, (4) Oligopotent, and (5) Unipotent. Each stem cell has a specific origin; the fertilized egg or zygote is a totipotent cell and...
Renewal of Intestinal Stem Cells01:23

Renewal of Intestinal Stem Cells

The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the goblet,...
General Transcription Factors01:30

General Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
The Bronchial Tree01:23

The Bronchial Tree

The human bronchi and bronchial tree play a crucial role in the respiratory system, facilitating the exchange of oxygen and carbon dioxide. Let's delve into the intricate structure and functions of these respiratory components.
The trachea, commonly known as the windpipe, is a tube that connects the larynx (voice box) to the bronchi. At a point called the carina, it bifurcates into two primary bronchi. The right primary bronchus is wider, shorter, and more vertical than the left primary...
Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

RNA-Binding Protein Trim71 Controls Epicardial Cell Migration.

Journal of cardiovascular development and disease·2026
Same author

A miR-200b-Filamin A axis drives epicardial contribution to cardiogenesis.

Scientific reports·2026
Same author

Mechanosensitive PIEZO2 channels shape coronary artery development.

Nature cardiovascular research·2025
Same author

Foxf1-mediated co-regulation of miR-495 and let-7c modulates epicardial cell migration and myocardial specification.

Cellular and molecular life sciences : CMLS·2025
Same author

Toll-like receptors ligand immunomodulators for the treatment congenital diaphragmatic hernia.

Orphanet journal of rare diseases·2024
Same author

Intramyocardial Sprouting Tip Cells Specify Coronary Arterialization.

Circulation research·2024

Related Experiment Video

Updated: May 10, 2026

Generation of 3D Whole Lung Organoids from Induced Pluripotent Stem Cells for Modeling Lung Developmental Biology and Disease
09:45

Generation of 3D Whole Lung Organoids from Induced Pluripotent Stem Cells for Modeling Lung Developmental Biology and Disease

Published on: April 12, 2021

Wt1-expressing progenitors contribute to multiple tissues in the developing lung.

Elena Cano1, Rita Carmona, Ramón Muñoz-Chápuli

  • 1Dept. of Animal Biology, Faculty of Sciences, Univ. of Málaga, 29071 Málaga, Spain. chapuli@uma.es.

American Journal of Physiology. Lung Cellular and Molecular Physiology
|July 2, 2013
PubMed
Summary

The embryonic mesothelium is a key source of lung mesenchymal cells, contributing to various lung structures. The Wilms

Keywords:
Wilms' tumor suppressor genecoelomic epitheliummesotheliumpulmonary development

More Related Videos

Studying Wnt Signaling During Patterning of Conducting Airways
13:00

Studying Wnt Signaling During Patterning of Conducting Airways

Published on: October 16, 2016

Precision-cut Mouse Lung Slices to Visualize Live Pulmonary Dendritic Cells
09:33

Precision-cut Mouse Lung Slices to Visualize Live Pulmonary Dendritic Cells

Published on: April 5, 2017

Related Experiment Videos

Last Updated: May 10, 2026

Generation of 3D Whole Lung Organoids from Induced Pluripotent Stem Cells for Modeling Lung Developmental Biology and Disease
09:45

Generation of 3D Whole Lung Organoids from Induced Pluripotent Stem Cells for Modeling Lung Developmental Biology and Disease

Published on: April 12, 2021

Studying Wnt Signaling During Patterning of Conducting Airways
13:00

Studying Wnt Signaling During Patterning of Conducting Airways

Published on: October 16, 2016

Precision-cut Mouse Lung Slices to Visualize Live Pulmonary Dendritic Cells
09:33

Precision-cut Mouse Lung Slices to Visualize Live Pulmonary Dendritic Cells

Published on: April 5, 2017

Area of Science:

  • Developmental biology
  • Regenerative medicine
  • Cell biology

Background:

  • Lung development originates from paired endodermal outgrowths enveloped by mesenchyme.
  • A portion of this mesenchyme originates from the mesothelium via epithelial-mesenchymal transition.
  • The developmental fate and role of mesothelium-derived mesenchyme in lung morphogenesis are not fully understood.

Purpose of the Study:

  • To investigate the contribution and developmental fate of mesothelium-derived mesenchyme in lung development.
  • To elucidate the role of the Wilms' tumor suppressor gene (Wt1) in lung morphogenesis.

Main Methods:

  • Utilized a transgenic mouse model (mWt1/IRES/GFP-Cre crossed with Rosa26R-EYFP reporter).
  • Tracked Wt1-expressing cell lineage using yellow fluorescent protein (YFP) for colocalization with differentiation markers.
  • Analyzed Wt1 knockout (Wt1-/-) embryos for developmental defects.

Main Results:

  • Wt1-expressing cells, originating from the embryonic mesothelium, significantly contribute to pulmonary endothelial and smooth muscle cells, bronchial musculature, tracheal and bronchial cartilage, and CD34+ fibroblast-like interstitial cells.
  • These Wt1-expressing mesenchymal cells exhibit broad differentiation potential, similar to surrounding splanchnopleural mesenchyme.
  • Wt1-/- embryos displayed severe pulmonary developmental defects, including fused lung lobes, reduced pleural cavities, and diaphragmatic hernia.

Conclusions:

  • Embryonic mesothelium-derived cells play a crucial and multifaceted role in lung morphogenesis.
  • The Wilms' tumor suppressor gene (Wt1) is essential for normal lung development.
  • Identified distinct origins for fibroblast-like interstitial cells, with Wt1-expressing cells contributing to specific populations and others potentially originating from circulating progenitors.