Microglial activation: measurement of cytokines by flow cytometry

Cytokine measurement is a prerequisite to understand the inflammatory state of the body. Quantitative analysis of cytokines by Western blotting and ELISA is a daunting task as these are time-consuming and error-prone protocols. With the advent of flow cytometry, the estimation of cytokines using the classical antigen-antibody reaction has become a popular choice with researchers/clinicians. Here, we describe a protocol for multiple cytokine analysis using flow cytometry.