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Patch Clamp

Patch Clamp

Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...

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Related Experiment Video

Updated: May 10, 2026

Isolation and Whole-Cell Patch-Clamp Recording of Hippocampal Microglia from Adult Mice

Isolation and Whole-Cell Patch-Clamp Recording of Hippocampal Microglia from Adult Mice

Published on: September 27, 2024

Patch clamp protocols to study ion channel activity in microglia.

Tom Schilling¹, Claudia Eder

¹St. George's, University of London, London, UK.

Methods in Molecular Biology (Clifton, N.J.)

|July 2, 2013

Summary

This study details how to use patch clamp techniques to analyze microglial ion channels. These methods help understand ion channel activity in microglia for physiological and disease research.

Area of Science:

Neuroscience
Cell Biology
Immunology

Background:

Microglia, the resident immune cells of the central nervous system, express diverse ion channels crucial for their function.
These ion channels are classified by ion selectivity (K+, H+, Na+, Ca2+, nonselective cation, Cl-) and activation modes (voltage-, Ca2+-, G protein-, swelling-, stretch-activated).

Purpose of the Study:

To summarize optimal patch clamp protocols for recording and analyzing microglial ion channel activity.
To provide methods for identifying specific ion channel types and investigating their properties in microglia.

Main Methods:

Patch clamp techniques, including perforated and whole-cell configurations, are presented as the primary methods.
Specific voltage protocols and defined solutions are utilized for precise ion channel identification and characterization.

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Published on: December 21, 2010

One-channel Cell-attached Patch-clamp Recording

One-channel Cell-attached Patch-clamp Recording

Published on: June 9, 2014

Related Experiment Videos

Last Updated: May 10, 2026

Isolation and Whole-Cell Patch-Clamp Recording of Hippocampal Microglia from Adult Mice

Isolation and Whole-Cell Patch-Clamp Recording of Hippocampal Microglia from Adult Mice

Published on: September 27, 2024

Application of a NMDA Receptor Conductance in Rat Midbrain Dopaminergic Neurons Using the Dynamic Clamp Technique

Application of a NMDA Receptor Conductance in Rat Midbrain Dopaminergic Neurons Using the Dynamic Clamp Technique

Published on: December 21, 2010

One-channel Cell-attached Patch-clamp Recording

One-channel Cell-attached Patch-clamp Recording

Published on: June 9, 2014

In vitro and in situ recordings are discussed for comprehensive analysis.

Main Results:

The perforated patch clamp technique is highlighted for studying microglial ion channel activity under physiological conditions, preserving cellular integrity.
Whole-cell patch clamp allows detailed biophysical and pharmacological investigation of identified ion channels.
The described protocols facilitate accurate recording and analysis of microglial ion channel function.

Conclusions:

Patch clamp techniques are essential for elucidating the roles of ion channels in microglial physiology and pathology.
Standardized protocols are crucial for reproducible and reliable studies of microglial ion channel activity.
This work provides a valuable resource for researchers investigating microglial function and therapeutic targets.