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Automated Microbial Diagnostics01:24

Automated Microbial Diagnostics

Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...

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Related Experiment Video

Updated: May 10, 2026

Systematic Approach to Identify Novel Antimicrobial and Antibiofilm Molecules from Plants' Extracts and Fractions to Prevent Dental Caries
08:20

Systematic Approach to Identify Novel Antimicrobial and Antibiofilm Molecules from Plants' Extracts and Fractions to Prevent Dental Caries

Published on: March 31, 2021

Lab-Test(®) 4: Dental caries and bacteriological analysis.

Francesca Cura1, Annalisa Palmieri, Ambra Girardi

  • 1Department of Histology, Embryology and Applied Biology, Centre of Molecular Genetics, CARISBO Foundation, University of Bologna, Bologna, Italy.

Dental Research Journal
|July 2, 2013
PubMed
Summary

A new Polymerase Chain Reaction Real Time test quantifies oral cariogenic bacteria, aiding in diagnosing dental caries. This helps in planning preventive treatments to reduce bacterial load and improve oral health.

Keywords:
Bacteriadental cariesreal-time polymerase chain reaction

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Area of Science:

  • Microbiology
  • Dental Science
  • Infectious Diseases

Background:

  • Dental caries is a prevalent multifactorial infectious disease globally.
  • It involves tooth demineralization due to bacterial acid production.
  • Key factors include specific bacteria, poor hygiene, immune response, diet, and tooth structure.

Purpose of the Study:

  • To develop a diagnostic test for quantifying common oral cariogenic bacteria.
  • To provide a patient infection profile for improved diagnosis and treatment planning.

Main Methods:

  • Utilized Polymerase Chain Reaction Real Time (PCR RT).
  • Employed specific primers and probes for amplifying bacterial genome sequences.
  • Quantified absolute and relative levels of oral cariogenic bacteria.

Main Results:

  • Developed a test for absolute and relative quantification of oral cariogenic bacteria.
  • The test provides a patient-specific infection profile.
  • Results aid in diagnosis and preventive treatment planning.

Conclusions:

  • The developed PCR RT test offers a valuable tool for assessing oral bacterial load.
  • This facilitates improved diagnosis and personalized preventive strategies for dental caries.
  • Reducing bacterial load is key to managing and preventing caries.