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Highly sensitive SERS quantification of the oncogenic protein c-Jun in cellular extracts
Luca Guerrini1, Elena Pazos, Cristina Penas
1Departamento de Ingenieria Electronica, Universitat Rovira i Virgili, Avda. Països Catalans 26, 43007 Tarragona, Spain.
Journal of the American Chemical Society
|July 3, 2013
Summary
A novel surface-enhanced Raman scattering (SERS) sensor detects the oncoprotein c-Jun at nanomolar levels. This advancement aids in understanding cancer mechanisms by monitoring c-Jun
Area of Science:
- Biochemistry
- Molecular Biology
- Nanotechnology
Background:
- Overexpression of the oncoprotein c-Jun, a bZIP transcription factor, is linked to human cancers.
- Understanding c-Jun's role in carcinogenesis requires sensitive detection methods.
Purpose of the Study:
- To develop a surface-enhanced Raman scattering (SERS)-based sensor for detecting the oncoprotein c-Jun.
- To quantify c-Jun at nanomolar concentrations.
Main Methods:
- Designed a c-Fos peptide receptor with an N-terminal thiophenol (TP) group.
- Utilized the c-Fos/c-Jun heterodimerization for sensing.
- Anchored the c-Fos receptor to a metal substrate via the TP group.
- Employed SERS to detect structural changes during heterodimerization.
Main Results:
- Successfully developed a SERS-based sensor for c-Jun detection.
- Achieved detection of c-Jun at nanomolar levels.
- Demonstrated the sensor's ability to identify c-Fos/c-Jun heterodimerization.
Conclusions:
- The developed SERS sensor provides a sensitive method for oncoprotein c-Jun detection.
- This technology can aid in cancer research by monitoring c-Jun activity.
- The sensing strategy leverages protein-protein interactions for molecular detection.
