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Basic fibroblast growth factor suppressed the enhancement of choline acetyltransferase activity induced by nerve
S Matsuda1, H Saito, N Nishiyama
1Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Abstract:
We investigated change of choline acetyltransferase (ChAT) activities in rat embryonic (16 days) septal neuron culture in treatment with basic fibroblast growth factor (bFGF) and nerve growth factor (NGF). Total ChAT activity increased by addition of bFGF, NGF, and bFGF plus NGF with dose-dependent manner. NGF showed much enhancement of specific ChAT activities per mg protein, but bFGF or bFGF plus NGF, respectively showed little or slightly enhanced ChAT activities. In histochemical studies with anti-ChAT antibody staining, cholinergic neurons in NGF-treated culture were stained more strongly than those in other conditioned cultures such as control, bFGF-treated, and bFGF plus NGF-treated cultures. These results suggest that bFGF enhances total ChAT activity but not cellular ChAT activity and further suppresses the enhancement of cellular ChAT activity induced by NGF in septal neuron culture.