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[Therapeutic effect of human bone marrow mesenchymal stem cell lysates on rat arthritis induced by collagen]
Xiao-Fang Cao1, Yong-Qi Wang, Zhi-Yong Li
1Hebei North University, Hebei Province, China.
Abstract:
Our previous work has shown that mesenchymal stem cells (MSC) have little therapeutic effect on rat arthritis induced by collagen. This study was aimed to further investigate whether the MSC lysates exhibit beneficial effects on rheumatoid arthritis. Aliquots of cell lysates from 1×10(7) human bone marrow MSC were intraperitoneally injected into collagen-induced arthritis (CIA) Wistar rats weekly for 4 consecutive weeks. Methotrexate at a dose of 1 mg/kg or normal saline was served as positive and negative controls respectively. On week 4 the symptom scores were recorded and the hind joints of the rats were pathologically examined and X-ray examination was performed. The results showed that on week 4, the symptom scores of the rats that received MSC lysates (6.87 ± 0.83) and MTX (6.44 ± 1.13) were significantly lower than that of control rats (7.33 ± 0.77, P < 0.01). Meanwhile, pathological examination on the involved ankle showed that the synovitis and arthritis scores of MSC lysates and control groups were 2.28 ± 0.48 and 2.28 ± 0.55 respectively, significantly higher than that of MTX treatment rats (0.71 ± 0.48, P < 0.05). However, X-ray examination on the ankle joints showed that the injury score of control rats was 4 ± 0.57, greatly higher than those from MSC lysates (2.71 ± 0.75) and MTX treatment groups (2.57 ± 0.78, P < 0.05 for both groups). It is concluded that MSC lysate infusion has beneficial effects on CIA rat, but the effectiveness seems inferior to MTX.
Insights
Mesenchymal stem cell (MSC) lysates showed therapeutic benefits for rheumatoid arthritis in rats, reducing symptom and X-ray scores. However, MSC lysates were less effective than methotrexate in this collagen-induced arthritis model.
Area of Science:
- Immunology
- Regenerative Medicine
- Pharmacology
Background:
- Mesenchymal stem cells (MSCs) have shown limited therapeutic effects in collagen-induced arthritis (CIA) rat models.
- Investigating MSC lysates offers an alternative approach to harness potential therapeutic benefits.
Purpose of the Study:
- To evaluate the efficacy of human bone marrow MSC lysates in treating collagen-induced arthritis (CIA) in Wistar rats.
- To compare the therapeutic effects of MSC lysates with methotrexate (MTX) and a saline control.
Main Methods:
- MSC lysates were administered intraperitoneally to CIA rats weekly for 4 weeks.
- Disease severity was assessed using symptom scores, pathological examination of ankle joints, and X-ray analysis.
- Methotrexate (1 mg/kg) and normal saline served as positive and negative controls, respectively.
Main Results:
- MSC lysate treatment significantly reduced overall symptom scores compared to controls (P < 0.01).
- Pathological examination revealed higher synovitis and arthritis scores in MSC lysate and control groups compared to MTX treatment (P < 0.05).
- X-ray analysis showed significantly lower injury scores in both MSC lysate and MTX groups compared to controls (P < 0.05).
Conclusions:
- MSC lysate infusion demonstrates beneficial effects in a rat model of collagen-induced arthritis.
- The therapeutic efficacy of MSC lysates appears to be inferior to that of methotrexate in this CIA model.
