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Updated: May 10, 2026

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
Published on: April 26, 2017
ExonSuite: algorithmically optimizing alternative gene splicing for the PUF proteins
Dilan Ustek1, Abraham Kohrman, Bogdan Krstic
1Grinnell College, Computer Science Department, USA. ustekdil@grinnell.edu
Abstract:
The stability of mRNA and its translation is a vital process necessary for proper protein production. The specificity of the regulation is controlled by specific RNA motifs and regulatory proteins. Pumilio/fem-3 mRNA-binding factor (PUF) proteins are usually used in regulating mRNA stability as well as translation. Here, we optimized a PUF protein target finder program to understand the natural diversity of RNA recognition by this family of proteins. ExonSuite is available to compile and run at https://github.com/dilanustek/ExonSuite.
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