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Related Concept Videos

Epigenetic Regulation01:37

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
Epigenetic Regulation01:46

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
Epigenetic Regulation01:46

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
Nucleosome Remodeling02:54

Nucleosome Remodeling

Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
Histone Modification02:32

Histone Modification

The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
Histone Modification02:32

Histone Modification

The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...

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Related Experiment Video

Updated: May 10, 2026

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
09:42

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Published on: September 7, 2017

DNA methylation regulated nucleosome dynamics.

Isabel Jimenez-Useche1, Jiaying Ke, Yuqing Tian

  • 1School of Chemical Engineering, Purdue University, West Lafayette, IN, USA.

Scientific Reports
|July 3, 2013
PubMed
Summary
This summary is machine-generated.

DNA methylation patterns influence nucleosome stability and conformation. This epigenetic modification impacts chromatin packaging and gene transcription by altering how DNA is wrapped around histone proteins.

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Last Updated: May 10, 2026

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Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors
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Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

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Area of Science:

  • Epigenetics
  • Structural Biology
  • Biochemistry

Background:

  • A known correlation exists between DNA methylation and nucleosome positioning.
  • The underlying mechanistic model for this correlation is not well understood.

Purpose of the Study:

  • To investigate how specific DNA methylation patterns affect nucleosome conformation and stability.
  • To elucidate the role of CpG dinucleotide repeats in nucleosome structure.

Main Methods:

  • Förster Resonance Energy Transfer (FRET) was used to analyze nucleosome conformation.
  • Small-Angle X-ray Scattering (SAXS) was employed to assess nucleosome stability.
  • The study examined CpG dinucleotide repeats at 10 bp intervals and a (CpG)5 stretch at the nucleosomal dyad.

Main Results:

  • CpG repeats at 10 bp intervals exhibited varied roles in nucleosome stability based on methylation state and location.
  • A (CpG)5 stretch at the nucleosomal central dyad did not alter conformation but showed methylation-dependent stability differences.
  • Significant conformational changes were observed between methylated and unmethylated nucleosomes.

Conclusions:

  • Nucleosome stability variations, influenced by sequence and epigenetic content (DNA methylation), can explain the correlation between nucleosome positioning and methylation patterns.
  • This research provides insights into DNA methylation's role in regulating chromatin packaging and gene transcription.