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Related Concept Videos

Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)01:19

2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

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When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...

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Related Experiment Video

Updated: May 10, 2026

Multimodal Nonlinear Hyperspectral Chemical Imaging Using Line-Scanning Vibrational Sum-Frequency Generation Microscopy
08:49

Multimodal Nonlinear Hyperspectral Chemical Imaging Using Line-Scanning Vibrational Sum-Frequency Generation Microscopy

Published on: December 1, 2023

Digital compressive chemical quantitation and hyperspectral imaging.

David S Wilcox1, Gregery T Buzzard, Bradley J Lucier

  • 1Purdue University, Department of Chemistry, West Lafayette, IN, USA.

The Analyst
|July 3, 2013
PubMed
Summary

Optimized binary (OB) filters accelerate Raman spectroscopy for analyzing liquid mixtures and solid powders. This method enables chemical quantitation even without pure component samples, using multivariate curve resolution (MCR).

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Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
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Published on: July 25, 2022

Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Chemometrics

Background:

  • Raman spectroscopy is a powerful technique for chemical analysis.
  • Quantifying mixtures and chemical imaging can be time-consuming.
  • Acquiring pure component spectra for training can be challenging.

Purpose of the Study:

  • To enhance the speed and applicability of Raman spectroscopy for mixture analysis.
  • To develop a method for chemical quantitation without pure component standards.
  • To demonstrate the utility of optimized binary (OB) filters in spectroscopic analysis.

Main Methods:

  • Implementation of digital compressive detection using optimized binary (OB) filters.
  • Application of multivariate curve resolution (MCR) for pre-processing spectral data.
  • Utilizing OB filters for the quantitation of liquid mixtures and chemical imaging of solid powders.

Main Results:

  • Significantly increased speed for Raman spectroscopic quantitation of liquid mixtures.
  • Enabled rapid chemical imaging of mixed solid powders.
  • Demonstrated successful mixture quantitation using MCR-generated OB filters without pure component training spectra.

Conclusions:

  • OB filters offer a substantial speed improvement for Raman spectroscopy applications.
  • MCR-based OB filter generation provides a robust method for mixture analysis, even with limited training data.
  • This approach expands the practical utility of Raman spectroscopy in compositional analysis and chemical imaging.