Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Neurogenesis and Regeneration of Nervous Tissue01:15

Neurogenesis and Regeneration of Nervous Tissue

In the CNS, neurogenesis, the birth of new neurons from stem cells, is limited to the hippocampus in adults. In other regions of the brain and spinal cord, neurogenesis is almost non-existent due to inhibitory influences from neuroglia, especially oligodendrocytes, and the absence of growth-stimulating cues. The myelin produced by oligodendrocytes in the CNS inhibits neuronal regeneration. Furthermore, astrocytes proliferate rapidly after neuronal damage, forming scar tissue that physically...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Challenges and prospects for malaria elimination in the Greater Mekong Subregion.

Acta tropicaยท2011
Same author

[Optimization of extraction procedure of tongmai granules by orthogonal design with pharmacodynamic index].

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medicaยท2011
Same author

Determinants of postoperative corneal edema and impact on goldmann intraocular pressure.

Corneaยท2011
Same author

Z-palatopharyngoplasty plus genioglossus advancement and hyoid suspension for obstructive sleep apnea hypopnea syndrome.

Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgeryยท2011
Same author

Efficient and selective photodimerization of 2-naphthalenecarbonitrile mediated by cucurbit[8]uril in an aqueous solution.

Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiologyยท2011
Same author

Quality assurance and quality improvement in U.S. clinical molecular genetic laboratories.

Current protocols in human geneticsยท2011

Related Experiment Video

Updated: May 10, 2026

Isolation, Expansion, and Nucleofection of Neural Stem Cells from Adult Murine Subventricular Zone
09:19

Isolation, Expansion, and Nucleofection of Neural Stem Cells from Adult Murine Subventricular Zone

Published on: June 14, 2024

Splicing factor TRA2B is required for neural progenitor survival.

Jacqueline M Roberts1, Hanane Ennajdaoui, Carina Edmondson

  • 1Department of Molecular, Cell, and Developmental Biology, University of California, Santa Cruz, California, 95064.

The Journal of Comparative Neurology
|July 3, 2013
PubMed
Summary

The RNA binding protein TRA2B (SFRS10) is crucial for mammalian brain development. TRA2B depletion during murine cortical neurogenesis causes neural progenitor cell death and disorganized brain structure.

Keywords:
SR proteinTRA2Bcerebral cortexdevelopmentneural progenitorsplicing

More Related Videos

Induction of Protein Deletion Through In Utero Electroporation to Define Deficits in Neuronal Migration in Transgenic Models
12:01

Induction of Protein Deletion Through In Utero Electroporation to Define Deficits in Neuronal Migration in Transgenic Models

Published on: January 12, 2015

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

Related Experiment Videos

Last Updated: May 10, 2026

Isolation, Expansion, and Nucleofection of Neural Stem Cells from Adult Murine Subventricular Zone
09:19

Isolation, Expansion, and Nucleofection of Neural Stem Cells from Adult Murine Subventricular Zone

Published on: June 14, 2024

Induction of Protein Deletion Through In Utero Electroporation to Define Deficits in Neuronal Migration in Transgenic Models
12:01

Induction of Protein Deletion Through In Utero Electroporation to Define Deficits in Neuronal Migration in Transgenic Models

Published on: January 12, 2015

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

Area of Science:

  • Molecular Biology
  • Neuroscience
  • Developmental Biology

Background:

  • Alternative splicing regulates protein expression and is vital for the central nervous system.
  • The RNA binding protein TRA2B (SFRS10) is known for its role in Drosophila development and mammalian embryogenesis.
  • The specific function of splicing factors like TRA2B in mammalian neurogenesis remains unclear.

Purpose of the Study:

  • To investigate the role of TRA2B in mammalian brain development, specifically during murine cortical neurogenesis.

Main Methods:

  • Utilized cortex-specific Tra2b mutant mice.
  • Analyzed TRA2B expression patterns in neural progenitors and cortical projection neurons during murine neurogenesis.

Main Results:

  • TRA2B is expressed in both neural progenitors and cortical projection neurons during murine cortical development.
  • TRA2B depletion in mutant mice led to increased apoptosis of neural progenitor cells.
  • Loss of TRA2B resulted in significant disorganization of the developing cortical plate.

Conclusions:

  • TRA2B is essential for the proper development of the cerebral cortex.
  • TRA2B plays a critical role in regulating neural progenitor cell survival and cortical structure formation.