Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 10, 2026

Drug-Induced Senescence in Liver Cells Promotes M2 Macrophage Polarization: Implications for Tyrosine Kinase Inhibitor-Associated Hepatotoxicity
09:32

Drug-Induced Senescence in Liver Cells Promotes M2 Macrophage Polarization: Implications for Tyrosine Kinase Inhibitor-Associated Hepatotoxicity

Published on: October 17, 2025

Triterpenoid pristimerin induced HepG2 cells apoptosis through ROS-mediated mitochondrial dysfunction.

Yan Guo1, Wei Zhang, Yan-Yan Yan

  • 1Wuhai Municipal People's Hospital, Wuhai, Inner Mongolia, People's Republic of China.

Journal of B.U.ON. : Official Journal of the Balkan Union of Oncology
|July 3, 2013
PubMed
Summary

Related Concept Videos

Electron Transport Chain: Complex I and II01:46

Electron Transport Chain: Complex I and II

The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Hemodynamic effects of synchronous and asynchronous independent lung ventilation with different levels of positive end-expiratory pressure and tidal volumes on unilateral lung injury in dogs].

Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases·2010
Same author

[Study on the immuno-effects and influencing factors of Chinese hamster ovary (CHO) cell hepatitis B vaccine among adults, under different dosages].

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi·2010
Same author

Assemblies of fluorine containing bent-shaped liquid crystal molecules studied by using scanning tunneling microscopy.

Journal of nanoscience and nanotechnology·2010
Same author

Carbon nanotubes induce secondary structure changes of bovine albumin in aqueous phase.

Journal of nanoscience and nanotechnology·2010
Same author

[Immunogenicity and protective efficacy of pertactin recombinants against Bordetella bronchiseptica challenge].

Wei sheng wu xue bao = Acta microbiologica Sinica·2010
Same author

[Analysis of the electrocardiographic findings in 288 patients with acute pulmonary thromboembolism].

Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases·2010

Pristimerin, a natural compound, effectively kills liver cancer cells (HepG2) by triggering programmed cell death (apoptosis). This process involves reactive oxygen species (ROS) and mitochondrial dysfunction.

Area of Science:

  • Pharmacology
  • Cell Biology
  • Biochemistry

Background:

  • Hepatocellular carcinoma (HCC) is a major global health concern.
  • Natural compounds offer potential therapeutic strategies for cancer treatment.
  • Pristimerin is a triterpenoid with known biological activities.

Purpose of the Study:

  • To investigate the anticancer effects of pristimerin on human hepatocellular carcinoma (HCC) HepG2 cells.
  • To elucidate the mechanisms underlying pristimerin-induced apoptosis and proliferation inhibition.

Main Methods:

  • Cytotoxicity assessed by MTT assay.
  • Apoptosis evaluated using Hoechst staining, Annexin V/PI, and caspase-3 activation.
  • Mitochondrial membrane potential (ΔΨm) and reactive oxygen species (ROS) measured by flow cytometry.

Related Experiment Videos

Last Updated: May 10, 2026

Drug-Induced Senescence in Liver Cells Promotes M2 Macrophage Polarization: Implications for Tyrosine Kinase Inhibitor-Associated Hepatotoxicity
09:32

Drug-Induced Senescence in Liver Cells Promotes M2 Macrophage Polarization: Implications for Tyrosine Kinase Inhibitor-Associated Hepatotoxicity

Published on: October 17, 2025

  • Protein expression analyzed via Western blot.

    • Main Results:

    • Pristimerin demonstrated potent dose-dependent cytotoxicity against HepG2 cells.
    • Apoptotic morphology, increased Annexin V-positive cells, and caspase-3 activation were observed.
    • Pristimerin induced ROS generation, mitochondrial membrane potential collapse, and cytochrome C release.
    • Changes in Bcl-2, Bax, and EGFR expression were noted, with downstream pathway inhibition.

    Conclusions:

    • Pristimerin effectively induces apoptosis in HepG2 cells.
    • Reactive oxygen species (ROS) play a critical role in pristimerin-mediated apoptosis.
    • Mitochondrial pathways are significantly involved in pristimerin's anticancer effects.