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Updated: May 10, 2026

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis
Published on: November 17, 2011
Lipidomics of essential fatty acids and oxygenated metabolites
Michel Lagarde1, Nathalie Bernoud-Hubac, Catherine Calzada
1Université de Lyon, UMR 1060 Inserm, IMBL, INSA-Lyon, Villeurbanne, France. michel.lagarde@insa-lyon.fr
Abstract:
Polyunsaturated fatty acids in mammals may be oxygenated into a myriad of bioactive products through di- and monooxygenases, products that are rapidly degraded to control their action. To evaluate the phenotypes of biological systems regarding this wide family of compounds, a lipidomics approach in function of time and compartments would be relevant. The current review takes into consideration most of the diverse oxygenated metabolites of essential fatty acids at large and their immediate degradation products. Their biological function and life span are considered. Overall, this is a fluxolipidomics approach that is emerging.
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