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Updated: May 10, 2026

Single-Copy Gene Locus Chromatin Purification in Saccharomyces cerevisiae

Single-Copy Gene Locus Chromatin Purification in Saccharomyces cerevisiae

Published on: November 17, 2023

Epigenetics in Saccharomyces cerevisiae.

Michael Grunstein¹, Susan M Gasser

¹University of California, Los Angeles, Los Angeles, California 90095, USA.

Cold Spring Harbor Perspectives in Biology

|July 3, 2013

Summary

This summary is machine-generated.

Silent chromatin in Saccharomyces cerevisiae, regulated by the SIR complex, offers insights into gene repression. Research advances molecular understanding of SIR-chromatin interactions and their physiological impacts.

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Last Updated: May 10, 2026

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Published on: November 17, 2023

In Vivo Monitoring of Transcriptional Activity During Metabolic Transition Using a Bioluminescent Reporter in Yeast

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Chromatin Immunoprecipitation (ChIP) of Histone Modifications from Saccharomyces cerevisiae

Chromatin Immunoprecipitation (ChIP) of Histone Modifications from Saccharomyces cerevisiae

Published on: December 29, 2017

Area of Science:

Molecular Biology
Genetics
Biochemistry

Background:

Saccharomyces cerevisiae serves as a model for studying heritable silent chromatin.
The SIR complex mediates gene repression through sequence-independent spreading, akin to heterochromatin in higher eukaryotes.
Histone mutations and genome-wide screens provide detailed insights into this system.

Purpose of the Study:

To advance the molecular understanding of the SIR-chromatin complex.
To elucidate the recognition mechanism between Sir3 and modified histone tails.
To explore physiological roles of silencing machinery components beyond transcriptional repression.

Main Methods:

Utilizing Saccharomyces cerevisiae as a model organism.
Employing genetic screens to identify mutations affecting silencing.
Applying biochemical and structural biology techniques to study the SIR-chromatin complex.

Main Results:

Detailed understanding of the SIR complex's role in heritable silent chromatin.
Insights into how Sir3 recognizes deacetylated histone H4 tails and demethylated histone H3 cores.
Demonstration of silencing machinery affecting physiological processes.

Conclusions:

The SIR complex in yeast is a powerful model for chromatin-based gene regulation.
Biochemical and structural studies are key to deciphering molecular recognition in silencing.
Components of the silencing machinery have broader physiological implications.