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Updated: May 10, 2026
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Measuring Spatial and Temporal Ca2+ Signals in Arabidopsis Plants
Published on: September 2, 2014
Live cell imaging of cytoplasmic Ca2+ dynamics in Arabidopsis guard cells
Smrutisanjita Behera1, Jörg Kudla
1Institute of Plant Biology and Biotechnology, University of Münster, 48149 Münster, Germany.
Cold Spring Harbor Protocols
|July 3, 2013
Abstract:
This protocol describes a classical method for measuring cytoplasmic Ca(2+) dynamics in Arabidopsis thaliana guard cells. The Förster (fluorescence) resonance energy transfer (FRET)-based genetically modified Ca(2+) indicator Yellow Cameleon YC3.6, under the control of the guard cell-specific promoter GC1, is used for Ca(2+) measurements.
