Measuring interstitial diffusion, convection, and binding parameters in mouse tumors
Cold Spring Harbor Protocols
|July 3, 2013
Summary
This study details a noninvasive method to measure extravascular parameters in mouse tumors. It uses fluorescence imaging and photobleaching techniques to assess diffusion, convection, and binding within tumor tissues.
Area of Science:
- Biomedical Engineering
- Molecular Imaging
- Cancer Research
Background:
- Noninvasive techniques are crucial for assessing tissue parameters in vivo.
- Understanding extravascular dynamics in tumors is vital for developing effective therapies.
Purpose of the Study:
- To describe a protocol for measuring extravascular parameters in mouse tumors.
- To quantify interstitial diffusion, convection, and binding in tumor microenvironments.
Main Methods:
- Infusion of a fluorescently labeled molecule into the tumor interstitium.
- Imaging using single-photon or multiphoton laser-scanning microscopy (MPLSM).
- Fluorescence recovery after photobleaching (FRAP) with spatial Fourier analysis, specifically multiphoton FRAP for diffusion coefficients.
Main Results:
- The protocol enables the measurement of key extravascular parameters.
- Multiphoton FRAP accurately quantifies interstitial diffusion coefficients.
- The method allows for detailed characterization of molecular transport and binding within tumors.
Conclusions:
- This noninvasive protocol provides a robust method for studying tumor microenvironment dynamics.
- The technique facilitates the assessment of diffusion, convection, and binding in mouse tumor models.
- This approach can aid in the evaluation of therapeutic efficacy and drug delivery strategies.
