Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Pulmonary Tuberculosis IV01:26

Pulmonary Tuberculosis IV

Tuberculosis, more commonly referred to as TB, is an infectious disease stemming from Mycobacterium tuberculosis. While it primarily impacts the lungs, TB can also affect other body areas. Given its severity and global impact, timely and accurate diagnosis is crucial for controlling its spread and improving patient outcomes.
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...
Pulmonary Tuberculosis III01:31

Pulmonary Tuberculosis III

Tuberculosis (TB) is a contagious infection primarily affecting the lung parenchyma but which can also affect other body parts. TB can be classified based on disease development, presentation, and the affected anatomical site.
The first classification is based on the development of the disease, and it includes the following categories:
Pulmonary Tuberculosis II01:28

Pulmonary Tuberculosis II

Tuberculosis, or TB, is a bacterial infectious disease caused by Mycobacterium tuberculosis. While its primary impact is on the lungs, leading to pulmonary tuberculosis, it can also affect various other organs, a condition referred to as extrapulmonary tuberculosis.
Here is a detailed explanation of its pathophysiology:
Transmission: The process begins when a person inhales droplet nuclei containing M. tuberculosis. These are typically released into the air when an individual with pulmonary or...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Novel base-initiated cascade reactions of hemiindigos to produce dipolar γ-carbolines and indole-fused pentacycles.

RSC advances·2022
Same author

Efficacy of BCG Vaccination Depends on Host Genetics.

Bulletin of experimental biology and medicine·2021
Same author

Use of Genetically Encoded Fluorescent Aptamers for Visualization of Mycobacterium tuberculosis Small RNA MTS1338 in Infected Macrophages.

Doklady. Biochemistry and biophysics·2020
Same author

Host genetics in susceptibility to and severity of mycobacterial diseases.

Tuberculosis (Edinburgh, Scotland)·2017
Same author

"Suppressor Factor" of Neutrophils: A Short Story of a Long-Term Misconception.

Biochemistry. Biokhimiia·2016
Same author

[The increase in receptor-mediated endocytosis of drugs in the composition of nanoparticles with the address fragment].

Biomeditsinskaia khimiia·2016

Related Experiment Video

Updated: May 10, 2026

An Experimental Model to Study Tuberculosis-Malaria Coinfection upon Natural Transmission of Mycobacterium tuberculosis and Plasmodium berghei
09:02

An Experimental Model to Study Tuberculosis-Malaria Coinfection upon Natural Transmission of Mycobacterium tuberculosis and Plasmodium berghei

Published on: February 17, 2014

Mycobacterium tuberculosis Transcriptome Profiling in Mice with Genetically Different Susceptibility to Tuberculosis.

T A Skvortsov1, D V Ignatov, K B Majorov

  • 1Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str., 16/10, Moscow, Russia, 117997.

Acta Naturae
|July 3, 2013
PubMed
Summary

This study reveals key gene expression changes in Mycobacterium tuberculosis during lung persistence. Upregulated genes in lipid metabolism and cell wall processes suggest adaptation strategies and potential drug targets for tuberculosis.

Keywords:
Mycobacterium tuberculosisRNA-seqnext generation sequencingtranscriptome in vivotuberculosis

More Related Videos

Imaging Mycobacterium tuberculosis in Mice with Reporter Enzyme Fluorescence
10:06

Imaging Mycobacterium tuberculosis in Mice with Reporter Enzyme Fluorescence

Published on: February 26, 2018

Preparation of Mycobacterium tuberculosis Culture Filtrate to Understand TB Pathogenesis
07:32

Preparation of Mycobacterium tuberculosis Culture Filtrate to Understand TB Pathogenesis

Published on: March 28, 2025

Related Experiment Videos

Last Updated: May 10, 2026

An Experimental Model to Study Tuberculosis-Malaria Coinfection upon Natural Transmission of Mycobacterium tuberculosis and Plasmodium berghei
09:02

An Experimental Model to Study Tuberculosis-Malaria Coinfection upon Natural Transmission of Mycobacterium tuberculosis and Plasmodium berghei

Published on: February 17, 2014

Imaging Mycobacterium tuberculosis in Mice with Reporter Enzyme Fluorescence
10:06

Imaging Mycobacterium tuberculosis in Mice with Reporter Enzyme Fluorescence

Published on: February 26, 2018

Preparation of Mycobacterium tuberculosis Culture Filtrate to Understand TB Pathogenesis
07:32

Preparation of Mycobacterium tuberculosis Culture Filtrate to Understand TB Pathogenesis

Published on: March 28, 2025

Area of Science:

  • Microbiology
  • Genomics
  • Pathogen Biology

Background:

  • Whole transcriptome profiling is crucial for understanding microbial behavior in vivo.
  • Mycobacterium tuberculosis (M. tuberculosis) is a major human bacterial pathogen causing tuberculosis.
  • In vivo transcriptome studies are essential for hypothesis generation and validation in infectious diseases.

Purpose of the Study:

  • To investigate genome-wide transcriptional changes of M. tuberculosis during persistent infection in mouse lungs.
  • To identify commonly upregulated genes (CUG) associated with disease progression in different host susceptibilities.
  • To explore potential roles of these genes in M. tuberculosis adaptation and identify new drug targets.

Main Methods:

  • Experimental infection of two mouse strains with M. tuberculosis.
  • Whole transcriptome profiling of M. tuberculosis from infected lung tissues at two time points.
  • Deep sequencing and comparative analysis of mycobacterial transcriptomes.

Main Results:

  • Identified 209 commonly upregulated genes (CUG) in M. tuberculosis during disease progression across both mouse strains.
  • Highlighted genes involved in lipid metabolism, cell wall, and cell processes as particularly interesting.
  • Observed potential shifts towards lipid and amino acid metabolism, anaerobic respiration, and immune modulation.

Conclusions:

  • The identified CUGs are likely involved in M. tuberculosis adaptation to host immune defenses.
  • Genes related to lipid metabolism and cell wall are promising candidates for novel tuberculosis drug development.
  • Transcriptome profiling provides valuable insights into pathogen survival mechanisms during chronic infection.