Related Experiment Video
Updated: May 10, 2026

Use of Bisection to Reduce Mitochondrial DNA in the Bovine Oocyte
Published on: July 6, 2022
Mitochondrial and DNA damage in bovine somatic cell nuclear transfer embryos
In-Sun Hwang1, Hyo-Kyung Bae, Hee-Tae Cheong
1College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University, Chuncheon 200-701, Korea.
Abstract:
The generation of reactive oxygen species (ROS) and subsequent mitochondrial and DNA damage in bovine somatic cell nuclear transfer (SCNT) embryos were examined. Bovine enucleated oocytes were electrofused with donor cells and then activated by a combination of Ca-ionophore and 6-dimethylaminopurine culture. The H2O2 and ˙OH radical levels, mitochondrial morphology and membrane potential (ΔΨ), and DNA fragmentation of SCNT and in vitro fertilized (IVF) embryos at the zygote stage were analyzed. The H2O2 (35.6 ± 1.1 pixels/embryo) and ˙OH radical levels (44.6 ± 1.2 pixels/embryo) of SCNT embryos were significantly higher than those of IVF embryos (19.2 ± 1.5 and 23.8 ± 1.8 pixels/embryo, respectively, p < 0.05). The mitochondria morphology of SCNT embryos was diffused within the cytoplasm. The ΔΨ of SCNT embryos was significantly lower (p < 0.05) than that of IVF embryos (0.95 ± 0.04 vs. 1.21 ± 0.06, red/green). Moreover, the comet tail length of SCNT embryos was longer than that of IVF embryos (515.5 ± 26.4 μm vs. 425.6 ± 25.0 μm, p < 0.05). These results indicate that mitochondrial and DNA damage increased in bovine SCNT embryos, which may have been induced by increased ROS levels.
More Related Videos
11:42Bottlenose Dolphin (Tursiops truncatus) Spermatozoa: Collection, Cryopreservation, and Heterologous In Vitro Fertilization
Published on: August 21, 2017
04:36A Simple Microaspiration Technique for Isolating Somatic Cells from Cryopreserved Equine Semen as Nuclear Donors for Cloning
Published on: December 19, 2025
Related Concept Videos
Animal Mitochondrial Genetics
Methods of Nuclear Reprogramming
In vitro Mutagenesis
DNA Damage can Stall the Cell Cycle
DNA Damage Can Stall the Cell Cycle
Introduction to Nuclear Reprogramming