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Related Experiment Video

Updated: May 10, 2026

Technique for Obtaining Mesenchymal Stem Cell from Adipose Tissue and Stromal Vascular Fraction Characterization in Long-Term Cryopreservation

Technique for Obtaining Mesenchymal Stem Cell from Adipose Tissue and Stromal Vascular Fraction Characterization in Long-Term Cryopreservation

Published on: December 30, 2021

Intestinal stem cells remain viable after prolonged tissue storage.

Megan K Fuller¹, Denver M Faulk, Nambirajan Sundaram

¹Department of Surgery, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.

Cell and Tissue Research

|July 4, 2013

Summary

Intestinal stem cells (ISCs) remain viable and retain their proliferative capacity for up to 30 hours when stored at 4°C. This finding suggests a potential therapeutic window for harvesting ISCs for regenerative medicine applications.

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Last Updated: May 10, 2026

Technique for Obtaining Mesenchymal Stem Cell from Adipose Tissue and Stromal Vascular Fraction Characterization in Long-Term Cryopreservation

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Published on: December 30, 2021

Isolating Stem Cells from Soft Musculoskeletal Tissues

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Published on: July 5, 2010

Intestinal Stem Cell Isolation and Culture in a Porcine Model of Segmental Small Intestinal Ischemia

Intestinal Stem Cell Isolation and Culture in a Porcine Model of Segmental Small Intestinal Ischemia

Published on: May 18, 2018

Area of Science:

Gastroenterology
Stem Cell Biology
Tissue Engineering

Background:

Intestinal stem cells (ISCs) are crucial for epithelial renewal and possess therapeutic potential.
The viability of ISCs during tissue storage is currently unknown.
Understanding ISC survival during storage is vital for their clinical application.

Purpose of the Study:

To determine the viability and proliferative capacity of ISCs after cold storage.
To investigate the impact of 4°C storage on intestinal tissue and ISC survival.
To assess the potential of using stored intestinal tissue for therapeutic purposes.

Main Methods:

Murine jejuna were stored at 4°C for 24, 30, or 48 hours.
Morphological integrity was assessed using light and electron microscopy.
ISC viability was evaluated by Lgr5-LacZ positivity and flow cytometry.
Enteroid formation capacity of isolated crypts was measured after storage.

Main Results:

Small intestinal crypts remained morphologically intact for up to 30 hours.
ISCs showed higher resistance to degeneration compared to other epithelial cells, including Paneth cells.
Isolated crypts retained enteroid-forming capacity after 24 hours of storage, with efficiencies above 80%.
Budding capability was retained even after 30 hours of storage, though efficiencies declined.

Conclusions:

ISCs remain viable and retain their proliferative capacity following short-term cold storage.
The majority of the intestinal epithelium undergoes degeneration and apoptosis during storage.
Cold storage of intestinal tissue may provide a therapeutic window for harvesting ISCs for regenerative medicine.