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Related Experiment Videos

Comparative studies on Megatrypanum trypanosomes from cervids.

M F Dirie1, S Bornstein, K R Wallbanks

  • 1Department of Biological Sciences, University of Salford, U.K.

Tropical Medicine and Parasitology : Official Organ of Deutsche Tropenmedizinische Gesellschaft and of Deutsche Gesellschaft Fur Technische Zusammenarbeit (GTZ)
|June 1, 1990
PubMed
Summary

Trypanosomes were found in Swedish reindeer and moose. Isoenzyme analysis suggests the reindeer isolates may represent a new species, distinct from other cervid trypanosomes.

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Area of Science:

  • Parasitology
  • Veterinary Medicine
  • Molecular Biology

Background:

  • Trypanosomes are protozoan parasites that infect a wide range of hosts, including cervids (deer family).
  • Previous studies have identified trypanosomes in North American cervids, but less is known about their prevalence and genetic diversity in European cervids.

Purpose of the Study:

  • To isolate and characterize trypanosomes from Swedish cervids (reindeer and moose).
  • To compare the genetic profiles (isoenzyme patterns) of Swedish isolates with North American isolates.
  • To investigate the potential for lectin-based differentiation of cervid trypanosomes.

Main Methods:

  • Parasite isolation via axenic culture and mammalian cell co-culture.
  • Morphological characterization of epimastigote and trypomastigote forms.

Related Experiment Videos

  • Isoenzyme electrophoresis for genetic comparison.
  • Surface carbohydrate analysis using lectins.

    • Main Results:

    • Trypanosomes were successfully isolated from two Swedish reindeer and one Swedish moose.
    • Morphological analysis showed similarities to known cervid trypanosomes.
    • Isoenzyme patterns of the Swedish moose isolate were similar to North American isolates.
    • The two Swedish reindeer isolates exhibited distinct isoenzyme patterns, suggesting a potential new species.
    • Lectin binding profiles differed between isolates, indicating potential for species-specific identification.

    Conclusions:

    • Trypanosome infections occur in Swedish cervids.
    • Genetic diversity exists among trypanosomes infecting reindeer across different continents.
    • The Swedish reindeer isolates may represent a novel trypanosome species.
    • Lectin-based methods offer a promising approach for differentiating trypanosome species within cervids.