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Brugia pahangi adults implanted into mice: a possible screen for filaricidal activity
D A Denham1, I T Midwinter, M J Shipley
1London School of Hygiene and Tropical Medicine, England.
Abstract:
Brugia pahangi adults grown in the peritoneal cavities of jirds (Meriones unguiculatus) were implanted into the peritoneal cavities of six inbred mouse strains to investigate this system as a screen for detecting filaricidal activity. The mice were given 15 adult B. pahangi and autopsied 35 days later. The recoveries of adult worms were 25%, 35%, 49%, 33%, 26% and 27% of the number implanted respectively for the MF1, TO, NIH, CBA, BALB/c and C3H/HE strains. There was great variation in the number of worms recovered from each strain of mouse. It is concluded that the variation in recoveries was so high that this system is not useful in detecting low level filaricidal activity.
Insights
This study evaluated a mouse model for detecting filaricidal activity. High worm recovery variation across mouse strains indicates the system is not suitable for screening low-level drug efficacy.
Area of Science:
- Parasitology
- Drug Discovery
- Animal Models
Background:
- Filarial infections pose a significant global health burden.
- Effective filaricidal drugs are crucial for disease control.
- Developing reliable screening systems is essential for identifying new treatments.
Purpose of the Study:
- To assess the utility of implanting Brugia pahangi adults into inbred mouse strains as a screening system for filaricidal activity.
- To evaluate the variability in worm recovery across different mouse strains.
Main Methods:
- Adult Brugia pahangi worms were surgically implanted into the peritoneal cavities of six inbred mouse strains (MF1, TO, NIH, CBA, BALB/c, C3H/HE).
- Mice received 15 adult worms and were autopsied after 35 days.
- Worm recovery rates were calculated for each mouse strain.
Main Results:
- Significant variation in adult worm recovery was observed among the tested mouse strains.
- Recovery percentages ranged from 25% to 49% of the implanted worms.
- Specific recovery rates: MF1 (25%), TO (35%), NIH (49%), CBA (33%), BALB/c (26%), C3H/HE (27%).
Conclusions:
- The high degree of variability in worm recovery across mouse strains renders this model unsuitable for detecting low-level filaricidal activity.
- Further refinement or alternative models are needed for effective screening of potential filaricidal compounds.