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Updated: May 10, 2026
Quantifiable and Inexpensive Cell-Free Fluorescent Method to Confirm the Ability of Novel Compounds to Chelate Iron
Published on: February 23, 2024
A pyrene-based highly selective turn-on fluorescent chemosensor for iron(III) ions and its application in living cell
Peter Kun Chung1, Shi-Rong Liu, Hsuan-Fu Wang
1Department of Applied Chemistry, National Chiao Tung University, Hsinchu, Taiwan, 300, Republic of China.
Abstract:
A new pyrene-based chemosensor (1) exhibits excellent selectivity for Fe(3+) ions over a wide range of tested metal ions Ag(+), Ca(2+), Cd(2+), Co(2+), Cu(2+), Fe(2+), Hg(2+), K(+), Mg(2+), Mn(2+), Ni(2+), Pb(2+), and Zn(2+). The binding of Fe(3+) to chemosensor 1 produces an emission band at 507 nm due to the formation of a Py-Py* excimer that is induced by Fe(3+)-binding. The binding ratio of 1-Fe(3+) was determined to be 1:1 from a Job plot. The association constant of 1-Fe(3+) complexes was found to be 1.27 × 10(4) M(-1) from a Benesi-Hildebrand plot. In addition, fluorescence microscopy experiments show that 1 can be used as a fluorescent probe for detecting Fe(3+) in living cells.
