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Updated: May 10, 2026
13:04
A Protocol for Analyzing Hepatitis C Virus Replication
Published on: June 26, 2014
Selecting and characterizing drug-resistant hepatitis C virus replicon
Inge Vliegen1, Leen Delang, Johan Neyts
1Rega Institute for Medical Research, Leuven, Belgium.
Methods in Molecular Biology (Clifton, N.J.)
|July 4, 2013
Abstract:
The current standard of care (SOC) for hepatitis C virus (HCV) genotype 1-infected patients consists of telaprevir or boceprevir in addition to pegylated interferon and ribavirin treatment. Other selective inhibitors of HCV replication are being developed. Drug pressure may, depending on the class of inhibitors, (rapidly) select for drug-resistant variants. Here we describe four different approaches to select in vitro for drug-resistant HCV subgenomic replicons.
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