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Updated: May 10, 2026

A Murine Model of Dengue Virus-induced Acute Viral Encephalitis-like Disease

A Murine Model of Dengue Virus-induced Acute Viral Encephalitis-like Disease

Published on: April 28, 2019

Testing antiviral compounds in a dengue mouse model.

Wouter Schul¹, Andy Yip, Pei-Yong Shi

¹Department of Infectious Diseases, Novartis Institutes for Biomedical Research, Emeryville, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)

|July 4, 2013

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A new mouse model using AG129 mice allows dengue virus (DENV) infection studies. This model aids in developing vaccines and antiviral therapies for this mosquito-borne flaviviral disease.

Area of Science:

Virology
Immunology
Infectious Diseases

Background:

Dengue fever is a significant global health threat caused by mosquito-borne flaviviruses.
Current treatments for dengue virus (DENV) infection are limited, with no approved vaccines or antiviral therapies.
Primates are susceptible to DENV, but a small animal model is crucial for research and development.

Purpose of the Study:

To establish and describe a rodent model for DENV infection.
To facilitate the development of vaccines and antiviral therapies for dengue.
To outline protocols for testing antiviral compounds in this model.

Main Methods:

Utilizing interferon-α/β and interferon-γ receptor-deficient (AG129) mice for DENV infection.
Establishing a protocol for inducing and managing DENV infection in AG129 mice.

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Last Updated: May 10, 2026

A Murine Model of Dengue Virus-induced Acute Viral Encephalitis-like Disease

A Murine Model of Dengue Virus-induced Acute Viral Encephalitis-like Disease

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Assays for the Identification of Novel Antivirals against Bluetongue Virus

Assays for the Identification of Novel Antivirals against Bluetongue Virus

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Isolation and Quantification of Zika Virus from Multiple Organs in a Mouse

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Implementing methods for administering antiviral compounds via oral gavage or parenteral injection.

Main Results:

Successfully established a functional DENV infection model in AG129 mice.
Demonstrated the utility of this model for evaluating antiviral compound efficacy.
Provided a reproducible protocol for DENV research in a small animal setting.

Conclusions:

The AG129 mouse model provides a valuable platform for dengue virus research.
This model is instrumental in the preclinical evaluation of novel dengue vaccines and antiviral drugs.
The described protocols support the advancement of dengue prevention and treatment strategies.