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Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

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Updated: May 10, 2026

Unravelling the Function of a Bacterial Effector from a Non-cultivable Plant Pathogen Using a Yeast Two-hybrid Screen
11:30

Unravelling the Function of a Bacterial Effector from a Non-cultivable Plant Pathogen Using a Yeast Two-hybrid Screen

Published on: January 20, 2017

Tip110 interacts with YB-1 and regulates each other's function.

Khalid Amine Timani1, Ying Liu, Johnny J He

  • 1University of North Texas Health Science Center, Fort Worth, TX 76107, USA.

BMC Molecular Biology
|July 5, 2013
PubMed
Summary
This summary is machine-generated.

Tip110 protein interacts with Y-box binding protein 1 (YB-1), influencing viral gene expression and RNA splicing. This complex formation mutually regulates their biological functions, expanding our understanding of Tip110's role.

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Area of Science:

  • Molecular Biology
  • Immunobiology
  • Virology

Background:

  • Tip110 is implicated in tumor immunobiology, gene expression, and protein turnover.
  • The complete biological functions of Tip110 are not fully understood.

Purpose of the Study:

  • To identify cellular proteins interacting with Tip110.
  • To investigate the functional relevance of the Tip110-YB-1 interaction.

Main Methods:

  • Immunoaffinity enrichment coupled with protein mass spectrometry.
  • Reporter gene assays for HIV-1 LTR transactivation.
  • In vivo splicing assays for CD44 minigene.

Main Results:

  • Identified 13 proteins complexed with Tip110, including Y-box binding protein 1 (YB-1).
  • Confirmed specific interaction between Tip110 and YB-1 involving their N-termini.
  • YB-1 enhances Tip110/Tat-mediated HIV-1 LTR transactivation.
  • Tip110 promotes exon 5 inclusion in CD44 alternative splicing.

Conclusions:

  • Tip110 and YB-1 form a complex.
  • The Tip110-YB-1 complex mutually regulates their biological functions.