Synergistic effect of dentinal tubule occlusion by nano-carbonate apatite and CO2 laser in vitro
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Published on: June 6, 2025
Ji Sun Kim1, Sun Young Han, Ho Keun Kwon
1Department of Preventive Dentistry and Public Oral Health, Research Center for Orofacial Hard Tissue Regeneration, Brain Korea 21 project, College of Dentistry, Yonsei University , Seoul, Republic of Korea.
Objective:
The aim of this study was to evaluate the occluding effects of a combination of dentifrice containing nano-carbonate apatite (n-CAP) and a CO2 laser on dentinal tubules, and to assess the acid resistance of the occluded dentinal tubules produced.
Background Data:
A number of experiments have been conducted recently to relieve the symptoms of dentin hypersensitivity (DH) using a laser in combination with desensitizing products.
Materials And Methods:
One hundred and twenty specimens with exposed dentinal tubules were divided into four groups: the control, n-CAP, laser, and combined groups. Thirty specimens in each group were reassigned into three different conditions: baseline, treatment for occluding dentinal tubules, and acid challenge (pH 4.0 acetate buffer solution for 3 min). At the end of each phase, all specimen surfaces were evaluated by scanning electron microscope (SEM).
Results:
The combined group had a significantly smaller mean dentinal tubule area than the control group, and the fewest reopened dentinal tubules after acid challenge.
Conclusions:
The combined therapy is a promising means of treating DH patients in the clinic.
