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Updated: May 10, 2026

Quantification of Visual Feature Selectivity of the Optokinetic Reflex in Mice

Quantification of Visual Feature Selectivity of the Optokinetic Reflex in Mice

Published on: June 23, 2023

A simple machine vision-driven system for measuring optokinetic reflex in small animals.

Yoshihiro Shirai¹, Kenta Asano, Yoshihiro Takegoshi

¹Laboratory for Neural Information Technology, Graduate School of Sciences and Engineering, University of Toyama, 3190 Gofuku, Toyama, Toyama 930-8555, Japan.

The Journal of Physiological Sciences : JPS

|July 5, 2013

Summary

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We developed an accessible optokinetic reflex (OKR) system using machine vision to monitor visual and motor functions. This reliable, low-cost setup accurately tracks pupils and detects pharmacological effects, aiding research in physiology and genetics.

Area of Science:

Neuroscience
Physiology
Biomedical Engineering

Background:

The optokinetic reflex (OKR) is a valuable tool for assessing visual and motor nervous system function.
Current OKR measurement methods are limited by the availability of specialized equipment and detailed DIY instructions.

Purpose of the Study:

To design and validate an easy-to-install, reliable, and low-cost system for measuring the optokinetic reflex (OKR).
To develop a computer program for automated pupil tracking and a mathematical method for estimating pupil azimuth.

Main Methods:

Utilized a low-cost machine vision platform with a user-friendly graphical interface for pupil detection.
Implemented a mathematical procedure to calculate pupil azimuth from visual location data.
Tested the system's performance in tracking mouse pupils and detecting pharmacological modulations of the cerebellar flocculi.

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PyOKR: A Semi-Automated Method for Quantifying Optokinetic Reflex Tracking Ability

PyOKR: A Semi-Automated Method for Quantifying Optokinetic Reflex Tracking Ability

Published on: April 12, 2024

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Last Updated: May 10, 2026

Quantification of Visual Feature Selectivity of the Optokinetic Reflex in Mice

Quantification of Visual Feature Selectivity of the Optokinetic Reflex in Mice

Published on: June 23, 2023

PyOKR: A Semi-Automated Method for Quantifying Optokinetic Reflex Tracking Ability

PyOKR: A Semi-Automated Method for Quantifying Optokinetic Reflex Tracking Ability

Published on: April 12, 2024

Main Results:

The system achieved a high success rate (~90%) in locating mouse pupils.
Pupil azimuth was estimated with high precision (~94%).
The system successfully detected changes in OKR gain following pharmacological interventions.

Conclusions:

The developed OKR measuring system is reliable, accurate, and accessible for research.
This system can facilitate behavioral assessments in various scientific disciplines, including physiology, pharmacology, and genetics.
The low-cost and user-friendly nature of the system promotes wider adoption in research settings.